Background:The members of the transforming growth factor-B superfamily, as the bone morphogenetic proteins (BMPs) subfamily and anti-Müllerian hormone (AMH), play a role during follicular development, and the bone morphogenetic protein-2 (BMP2), AMH, and THY1 are expressed in ovaries.Aim:This study was designed to define whether or not the expressions of these proteins in human cumulus cells (CCs) can be used as predictors of the oocyte and embryo competence.Settings and Design:The study included nine female patients who were diagnosed as idiopathic infertility, aged 25–33 years (median 30 years) and underwent Assisted Reproductive Technologies.Materials and Methods:The CCs from 60 oocyte–cumulus complexes obtained from the nine patients were evaluated with immunofluorescence staining in respect of BMPs, AMH and THY1 markers. The CCs surrounding the same oocytes were evaluated separately according to the oocyte and embryo quality.Statistical Analysis:Quantitative data were statistically analyzed for differences using the two-sided Mann–Whitney U test (P < 0.05).Results and Conclusions:Significant differences in immunofluorescence staining were observed in oocyte quality and embryo quality for the BMP2 only (P < 0.05). No significant differences were observed for AMH or CD90/THY1.Conclusion:These results demonstrated that there is a significant difference in the expression of BMP2 in the CCs of good quality oocytes and subsequently a good embryo.

No abstract

Objective This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p -value and highest number of times found count. Conclusion Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy.

A Am ma aç ç: : Bu yazıda tüpbebek uygulamalarında gebelik elde etmek için elde edilmesi gereken optimum oosit sayısı ve elde edilen oosit sayısı-nın farklı yaş gruplarında başarıya etkisi araştırıldı. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Ege Üniversitesi Aile Planlaması ve Kısırlık Araştırma ve Uygulama Merkezinde 2008-2013 yılları arasındaki 6405 IVF siklusu retrospektif olarak tarandı. Tüm sikluslar yaş gruplarına göre 30 yaş altı (n:1870), 30-34 (n:2074), 35-39 (n:1920) ve 40 (n:541) yaş üstü olarak 4 gruba ayrıldı. Temel özellikleri, tedaviye cevap ve sonuçlar kaydedildi. B Bu ul lg gu ul la ar r: : Yaşla birlikte kullanılan ilaç dozu artmakta, indüksiyon süresi uzamakta ve oosit sayısı azalmaktadır.yaş grupları elde edilen oositler açısından karşılaştırıldığında <5 oosit elde edilenlerde yaştan bağımsız gebelik başarısı düşüktür. ≥20 oositte ovarian hiperstimülasyon riski nedeniyle transfer iptalleri artmaktadır. S So on nu uç ç: : Elde edilen 10-15 oosit aralığı başarı ve yan etki açısından en avantajlı gö-rünen sayı olarak değerlendirilmiştir.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : İnfertilite; oosit; gebelik; yardımcı üreme teknikleri A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : We investigated the optimal number of oocytes to be obtained to achieve pregnancy in vitro fertilization cycles in different age groups. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : 6405 IVF cycles were retrospectively screened between 2008-2013 at Ege University Family Planning and Infertility Research and Treatment Center. All cycles were divided into 4 groups according to age groups: below 30 years old (n: 1870), 30-34 (n: 2074), 35-39 (n: 1920) and over 40 (n: 541) years. Basic characteristics, treatment response and results were recorded. R Re es su ul lt ts s: : Dosage of gonadotropins and duration of treatment were increasing with age, as the number of the obtained oocytes were decreasing. When the number of oocytes were <5; pregnancy rates were low in all groups. Cycle cancellation rates were increasing in ≥20 oocyte group due to the risk of OHSS. C Co on nc cl lu us si io on n: : The 10-15 oocyte range was the most advantageous number in terms of success and side effects.

No abstract