A highly selective, sensitive and accurate HPLC method has been developed and validated for the estimation of three fluoroquinolones (FQs) viz., gatifloxacin (GFC), sparfloxacin (SFC) and moxifloxacin (MFC) with 500 microL human plasma using levofloxacin (LFC) as an internal standard (IS). The sample preparation involved simple liquid-liquid extraction of GFC, SFC, MFC and IS from human plasma with ethyl acetate. The resolution of peaks was achieved with phosphate buffer (pH 2.5)-acetonitrile (80:20, v/v) at a flow rate of 1 mL/min on a Kromasil C(18) column. The total chromatographic run time was 18.0 min and the simultaneous elution of GFC, SFC, MFC and IS occurred at approximately 10.8, 12.8, 17.0 and 6.0 min, respectively. The method proved to be accurate and precise at linearity range of 100-10,000 ng/mL with a correlation coefficient (r) of > or =0.999. The limit of quantitation for each of the FQs studied was 100 ng/mL. The intra- and inter-day precision and accuracy values found to be within the assay variability limits as per the FDA guidelines. The developed assay method was applied to a pharmacokinetic study in human volunteers following oral administration of 400 mg GFC tablet.

No abstract

No abstract

The effect of CaUistemon lanceolatus (Syn. C. citrinus curtis; Family: Myrtaceae) leaf oil was studied for the antinociceptive and anti-inflammatory activity in experimental animals. C. lanceolatus, 25-100 mgkg administered orally for 3 days exhibited graded dose response equivalent to 21.95%-89.90% protection in the tail flick latent test in rat. The C. lanceolatus oil (50 and 100 mgkg, given orally for 3 days) was effective in hot plate reaction time (64.05% and 112.97%, p< 0.01 and p< 0.001), analgesymeter induced mechanical pain (28.17% and 54.42%, p C 0.01 and p C 0.001) and acetic acid-induced writhing (26.68% and 51.79%, p < 0.5 and p < 0.05) in mice. The oil of C. lanceolatus potentiated the analgesic activity with pentazocine (10 mgkg, i.p.) and aspirin (25 mgkg, i.p.). In the carrageenan-induced paw edema C. lanceolatus oil (50 and 100 mglkg, given orally for 3 days) decreased paw volume significantly (26.68% and 51.79%) and dose dependent anti-inflammatory activity in 1-3 hour time interval and potentiated with nimesulide (50 mgkg, p.0.). In summary, this study demonstrates that leaf oil of C. lanceolatus has significant antinociceptive and anti-inflammatory activity.