Background<br />Dopamine is an important neurotransmitter that plays a role in the pathogenesis of anxiety and depression. Dopamine secretion occurs when there is a pleasurable stimulus. Blood dopamine levels have the potential to be developed as biomarkers of depression or anxiety, but previous studies related to the relationship between dopamine levels and levels of anxiety and depression are still controversial. The purpose of this study was to determine the correlation between anxiety and depression score with dopamine level in young adults. <br /><br />Methods<br />This was an observational cross sectional study. A total of 43 subjects aged 18 to 40 years were recruited by consecutive non-random sampling according to inclusion and exclusion criteria. Anxiety and depression were assessed using the Beck anxiety inventory and Beck depression inventory-II questionnaire. Dopamine plasma level was measured using the enzyme-linked immunosorbent assay (ELISA). The Spearman rho correlation test was used to analyze the data and p&lt;0.05 was considered significant.<br /><br />Results <br />The median dopamine levels in respondents with mild, moderate and severe anxiety were respectively: 77.79; 63.43; 62.51 ng/mL (p=0.043). The median dopamine levels in respondents with reasonable depression, mood disorder, moderate and severe depression were respectively: 79.2; 61,32; 62,51; 60.24 (p=0.001). Correlation test results showed a weak correlation between dopamine and anxiety (r=- 0.310) and a moderate correlation for depression (r=-0.505).<br /><br />Conclusion<br />Mild anxiety and depression were associated with elevated dopamine level in young adults. Further studies are required to confirm the role of dopamine metabolism in anxiety and depression in young adults.

Each year more than 41,000 blood donations are needed every day and 30 million blood components are transfused. Blood products that can be transfused include Packed Red Cells (PRC), Whole Blood (WB), Thrombocyte Concentrate (TC), Fresh Frozen Plasma (FFP). Monitoring Hemoglobin (Hb) after transfusion is essential for assessing the success of a transfusion. The time factor after transfusion for Hemoglobin (Hb) examination needs to be established, analyze to judge the success of a blood transfusion which is performed. The aim of this study was to analyze the differences in changes of hemoglobin between 6-12 hours, and 12-24 hours after-transfusion. This study was retrospective observational using secondary data. The subjects were patients who received PRC, and WBC transfusion. At 6-12, and 12-24 hours after-transfusion, hemoglobin, RBC, and hematocrit were measured. Then the data were analyzed by unpaired t-test. The collected data included the results of the Hb pre-transfusion, 6-12, and 12-24 hours after-transfusion. The subjects of this study were 98 people. The administration of transfusion increased by 10-30% in hemoglobin concentration at 6-12 hours after-transfusion. While at 12-24 hours after-transfusion, hemoglobin after-transfusion increased 15-37% from the baseline. Hemoglobin values were not different at any of the defined after-transfusion times (p = 0.76 (p>0.05)). Hemoglobin values were not different at 6-12 hours, and 12-24 hours after-transfusion.    Keywords: Hemoglobin, measurement, after-transfusion

Deficiency of vitamin D in the world is high. Vitamin D deficiency has numerous negative effects. The purpose of this study was to determine the proportion of vitamin D deficiency in male adolescents in Yogyakarta Indonesia. This research is a cross-sectional study. The height, weight, physical activity, lipid profile laboratory data and vitamin D levels of subjects were recorded in the study. Body mass index (BMI) is calculated as body weight divided by height. Moderate exercise in physical is determined as enough activity for 150 minutes in week for moderate exercise and 75 minutes in week for strenuous exercise. Dyslipidaemia is diagnosed as an abnormal lipid level of three criteria: Low-Density Lipoprotein (LDL) ≥140 mg/dl, High-Density Lipoprotein (HDL) <40 mg/dl, and triglyceride ≥150 mg/dl.Vitamin deficiency was determined using the enzymes linked immunoassay method by measuring the activity of 25hydroxy vitamin D using serum samples of subjects. A total of 60 male adolescents aged 19-25 years participated in the study. A total of 30 subjects (50%) had obesity with an average BMI of 29.65 kg/m2. A total of 38 subjects had increased dyslipidaemia, with the highest proportion having low HDL (15%). A total of 39 subjects (65%) had had sufficient physical activity. The proportion of vitamin D deficiency was experienced by 26 subjects (43.3%) while the proportion of vitamin D insufficiency was experienced by 31 subjects (51.7%). Statistical analysis showed there was no correlation between the occurrence of vitamin D deficiency and obesity, dyslipidaemia and physical activity (Χ 2 = 0.778, p = 0.6779; Χ 2 = 1.8, p = 0.4065; Χ 2 = 0.087, p = 0.575). Proportion of vitamin D deficiency and insufficiency was quite high among male adolescents but not related to physical activity. Interventions are needed to treat conditions of vitamin D deficiency and insufficiency.

Introduction: Around twenty five million people in United State have at least one complications of atherosclerosis such as cardiovascular disease. Therefore screening in atherosclerotic process is important. Atherosclerosis is a multistage process that begins with damage to the endothelium due to LDL exposure. Angiography Coronary is the primary diagnostic tool for atherosclerosis. Imaging, as one way of non-invasive diagnosis, have limitation in sensitivity, specificity, and cost. Proteome urine analysis is alternative for atherosclerosis diagnostic. Objective: The aim of this study was analyse diagnostic performance of urine proteome among white rat (Rattus Novergicus strain wistar) induced by atherogenic diet. Material and methods: This study was quasi experimental in laboratory with randomized posttest only for control group design. The rat samples were divided to two groups i.e. group with atherogenic diet and non-atherogenic diet. Each group consisted of 10 rats. The treatment was done for 15 weeks. Urine sample collection was performed in week 16 of treatment. Urine proteome, collagen type I, was analyzed by ELISA to obtain biomarkers of atherosclerosis. Then determined sensitivity and specificity of the proteome urine analysis was compared with histopathology findings of the aortic arch and its branches. Result: In week 16, the researchers collected sample from 16 rats i.e. 11 rats with atherogenic diet and 5 ratswith non-atherogenic diet. Urine proteome, collagen type I, had area under the receiver operator with the characteristic curve of 98.2% (95% IK 92.6%-100%), p 0,003. The bestcut offis 0.9025 ng/ml with sensitivity of 100% and specificity of 90.9%. Conclusion: Urine proteome analyse, collagen type I, had high accuracy for atherosclerosis diagnostic.

Obesity is still a national problem. The prevalence of obesity is raising with increasing individual income. Various innovations in the field of sports do not increase the desire and tendency to exercise. Personal motivation is needed to lose weight which is directly beneficial to health. The presence of stigma in obese women may increase the tendency to exercise. The purpose of this study was to investigate the desire to exercise in obese adult women. This report is the second report of the ongoing research entitled "The Effect of Obesity towards Low Physical Activity" funded by the Research and Community Service Directorate, General Directorate of Research Strengthening and Developing, Ministry of Research, Technology, and Higher Education, Indonesia. This study took data from 10 obese female subjects and 12 non-obese female subjects with age matching. Data was collected through a questionnaire and analyzed using the Mann Whitney test. Data analysis employed the Medcalc program and was declared significant with p <0.05. The analysis revealed that there was no difference in median sports motivation scores between groups (60.5 vs 62, p = 0.407). The results of this study indicate that stigma in obese women has no effect on the desire to exercise.