A near infrared spectrophotometric (NIR) method was examined to confirm its applicability for measuring the major constituents in raw milk.The samples tested were collected at the National Institute of Animal Industry.The reference methods used were official analytical methods (Gerber,macro-Kjeldahl, Lane-Eynon and the standard gravimetric method) ,and the NIR instrument used was a Model 6350 (Neotec

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Lactobacillus bulgaricus incorporated exogenous guanine only into guanine nucleotides and not into adenine nucleotides. Lactobacillus bulgaricus lacks the ability to interconvert adenine nucleotides and guanine nucleotides. Biosynthesis of pyrimidine nucleotides from orotic acid was depressed by addition of guanosine monophosphate or adenosine monophosphate. Some step in pyrimidine biosynthesis from orotic acid may be negatively regulated by the intracellular amount of purine nucleotides. Lactobacillus bulgaricus could not grow in milk from which orotate was removed. This indicates that the activity of de novo pyrimidine synthesis is very low in this organism. When the intracellular amount of purine or pyrimidine base was limited, ribonucleic acid synthesis was markedly depressed, resulting in cell elongation, whereas deoxyribonucleic acid synthesis was not so much affected.

An extremely UV-resistant stage was found to appear only temporarily in the early phase of germination of B. subtilis spores. Kinetic analysis of the course of germination with special reference to ultraviolet and heat resistance revealed that the germinating spores remain at this transient stage for one to six minutes depending on the condition of germination, and Transient UV-resistant Stage in Spore Germination 281

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