Evaluate the serum concentration of testosterone hormone in some healthy adult’s men according to their characteristics of anthropometric measurements. This study included ninety-eight volunteer’s adult’s men, their ages (20-50) years, the blood samples were taken from subjects at the morning during 8:30-10:30 o’clock. ELISA kit was used to assay the serum level of testosterone hormone and the anthropometric measurements have been done such as; body mass index (BMI), waist circumference (WC) and percentage of body fat (BF%) by a special formula and the subjects were assorted depending on classes of anthropometric measurements. The results showed a significant decrease in serum level of testosterone hormone (p<0.05) in obese groups compared with the leaner groups. The class of the anthropometric measurements predicts the state of testosterone hormone level.

Ionized calcium represents the portion of total calcium and has biologically active; this study has been debated whether the calcium homeostasis status in correlated with anti-Mullerian hormone level how does influence on the reproductive ability in some pre-menopausal women. Eighty volunteer women non-smoking (20-35) years enrolled in this study,some of them (50 women) with primary infertility considered as study group,while the other 30 volunteer parous women considered as control group,non-fasting blood samples were taken from subjects during October 2015 till may 2016. ELISA kit was used to assay the serum level of anti-Mullerian and parathyroid hormone,whereas colorimetric method was used to determine serum total calcium,albumin and inorganic phosphorus,while ionized calcium is calculated by special equation depending on the total calcium and albumin level. Independent T-test analysis was used to determine any significant difference for investigated parameters between control and study group,linear regression analyses were in addition used to explore the association between ionized calcium and anti-Mullerian hormone (AMH) level in both control and infertile groups.infertile group have significantly p<0.001 lower mean serum ionized calcium level (4.31±0.61mg/dl) than in the control group (5.57±0.45mg/dl) accompanied by significantly increase in parathyroid hormone level (94.2±32.2ng/dl) in study groupin comparison with control group where they showed (92.1±22.2 ng/dl) mean concentration,in addition those infertile women had higher significantly (p<0.05) albumin level compared with those in control group,in addition to that,mean serum phosphorus level showed a significantly higher (p<0.01) in study group compared with its level in the control group,however infertile women revealed significantly decrease mean level in anti-Mullerian hormone (2.59±1.38ng/dl)compared with the hormone level in the control group (4.33±1.00ng/dl).As such results of linear regression analyses revealed a significant positive correlation between ionized calcium concentration and AMH level (r=0.58,p=0.000,n=40) in control group, as well,there was a significant strong positive correlation was seen between serum ionized calcium and AMH level for the infertile group (r=0.70,p=0.000,n=50).any physiological process including reproductive ability may need in one way or another to calcium metal. So,it's necessary maintain the concentration of blood calcium within stable normal range to ensure the work of most biological system properly including reproductive capacity and any disturbance in calcium homeostasis may have been altered the ovarian reserve which may in turn,reduce reproductive capacity.

Ionized calcium has a biological activity, this study has been debated whether the status of calcium homeostasis in correlated with anti-Mullerian hormone level how does influence the reproductive ability in some pre-menopausal women. The current study was conducted in the college of science for women during the period from February 2015 to May 2016, study population was involved eighty volunteer women (20-35) years enrolled in this study, fifty women with primary infertility considered as a study group, while the other 30 volunteers parous women considered as a control group. ELISA kit was used to assay the serum level of anti-Mullerian and parathyroid hormone, whereas colorimetric technique was applied to evaluate the total calcium, albumin, and inorganic phosphorus serum level, while ionized calcium is achieved by special equation depending on the total calcium and albumin level. Independent T-test analysis was applied to specify any significant variation for investigated parameters between control and study group, linear regression analyses were used to explore the association between ionized calcium and anti-Mullerian hormone (AMH) level in both control and infertile groups. The results revealed that infertile group have significantly (p<0.001) lower mean serum ionized calcium level(4.31±0.61mg/dl)than the control group(5.57±0.45mg/dl) accompanied by significantly (p<0.001) increase in parathyroid hormone level (49.2±32.2 ng/dl) in a study group in comparison with a control group whose reported (92.1±22.2ng/dl) mean concentration ,infertile women revealed significantly decrease mean level in anti-Mullerian hormone (2.59±1.38ng/d)  compared with  the control group (3.44±1.00ng/dl), linear regression analyses revealed a significant positive relation between's ionized calcium concentration and AMH level (r=0.58, p=0.000, n=40) in the control group, as well, there was a significant strong positive connection was seen between serum ionized calcium and AMH level for the infertile group (r=0.70, p=0.000, n=50),our conclusion suggested that any physiological process including reproductive ability may need in one way or another to balance in the availability of ionized calcium.

Prostate cancer is a significant disease in men, and a large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be established, which could aid in the early detection of the disease which is crucial for successful treatment. The first objective of this study was detection of allele frequencies of 12 Y-chromosome short tandem repeat loci from Iraqian prostate cancer patients and normal control males. The second objective was to evaluate the importance of these loci to develop prostate cancer. Blood samples were collected from 70 patients unrelated males living in middle and south of Iraq. FTA® Technology was utilized to extract DNA from blood collected on FTA™ paper. Post PCR amplification was detected by using ABI Prism1 3130xl Genetic Analyzer 16-capillary array system, with POP-7™ Polymer and Data Collection Software, Genemapper version 3.5 software. A higher incidence of disease was found among males who had either allele 10 of DYS391 or allele 9 of DYS459. It is likely that Iraqi males who belong to Y-lineages with either allele 10 or allele 9 are more susceptible to develop prostate cancer, while those belonging to lineages with allele 17 of DYS456 and DYS19 are more resistant to the disease. This study shows the influence of genetic elements on prostate cancer, and it seems that DYS391 and DYS459 locus comprising with other loci have the potential to be used as a screening method for prediction of susceptibility to prostate cancer in Iraqi population.