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Introduction: Antibiotics are prescribed for treating bacterial infections. However, Excessive use of antibiotics leads to the development and spread of multidrug resistant bacteria, currently regarded as a global public health crisis threatening our ability to treat common infectious diseases, resulting in prolonged illness, disability and death. Aim of the work:To analyze the use and prescribing patterns of antimicrobials by physicians at Ain-Shams University Specialized Hospital (ASUSH) for inpatients among different infections and to determine the prevalence of different types of bacterial resistance among hospital infections. Subjects and methods: A cross-sectional study was conducted at (ASUSH). A total of 339 microorganisms isolated from several samples types were identified and studied using VITEK® 2 system. Physicians' attitudes and knowledge were assessed using a questionnaire about antibiotic resistance problem. Results: Among (339) bacterial isolates Klebsiella pneumoniae was the most common isolate n=92 (27.1%), followed by E-coli n=60 (17.7%), Staph coag -ve (SCONs) n= 43 (12.7%) and Methecillin resistant staphylococcus aureas (MRSA), Pseudomonas aeruginosa and Acinetobacter baumanni n=33 (9.7%)