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Objective:The leaves of Lantana camara L., Eclipta prostrata (L.) L. and Cosmos caudatus Kunth. are rich in flavonoids, saponins and essential oils. This study was carried out to determine the antiplatelet effect of the three plant extracts on ADP-induced platelet aggregation. Methods: The samples consisted of clopidogrel as positive control, aquadest as negative control and test extracts with concentrations of 40, 60, 80 and 100 mg/ml. Platelet Rich Plasma (PRP) was added for each extract concentration. The absorbance test of the samples was measured at 600 nm, before and after the addition of 20 μL adenosine diphosphate (ADP) 5 mM. After ADP added, then measured again the plasma absorbance was measured of the same wavelength. Antiplatelet aggregation activity was determined by calculating plasma absorbance before and after addition of ADP. Results: The results showed that the inhibitory percentage of platelet aggregation with extract concentrations of 100, 80, 60 and 40 mg/ml were Lantana camara L. leaf extract (91.17%; 73.27%; 72.86% and 37.15%), Eclipta prostrata (L.) L. leaf extract (74.55%; 65.60%; 48.00% dan 39.08%) and Cosmos caudatus Kunth. Leaf extract (59.17%; 52.56%; 14.25% dan 13.82%), respectively. The test extracts inhibited in vitro ADP-induced platelet aggregation with IC 50 value were as follows: 47.09 mg/ml (Lantana camara L), 59.02 mg/ml (Eclipta prostrata (L.) L.) and 87.26 mg/ml (Cosmos caudatus Kunth.) Conclusion: The present work demonstrated that the three extracts could inhibit in-vitro ADP-induced platelet aggregation and the best potency was shown by Lantana camara L leaf extract.

Introduction: Zephyranthes candida plants or known as chocolate flowers are usually only used as ornamental plants, many people in Indonesia do not know that this plant has medicinal properties. The purpose of this study was to determine total phenol levels, and total flavonoids and determine the antioxidant activity of chocolate flower plants. Methods: This study was an experimental study using cacao flower leaves, ethanol, aqua dest, ABTS, gallic acid, quercetin, AlCl3, Folin-Ciocalteu reagent, Na2CO3, FeCl3, sodium acetate, and Vitamin C. Samples of cacao flower leaves (Zephyranthes candida) were collected from Leles, Garut Regency, West Java. The material taken is collected and then sorted wet to separate the plant parts that are not needed, then washed with running water until clean. Then dried in a drying cabinet at a temperature of 40-50°C to dry. Furthermore, dry sorting is carried out to separate foreign objects that enter during drying. The dried samples were mashed using a blender. Then the simplicia powder is stored in a tightly closed container at room temperature and is ready to be extracted. Then the extract will be analyzed to determine the total phenol content, flavonoid content, antioxidant activity, and thin layer chromatography. Results: The results of the determination of total phenol and total flavonoid levels and the antioxidant activity of brown flower leaves showed that the total phenol content of the brown flower ethanol extract was 20.5803 ± 0.15 mgGAE/gram. And the total flavonoid content of the brown flower ethanol extract was 1.8411 ± 0.0641 mgQE / g. The test results of the antioxidant activity of brown flower leaves showed that these plants had a very strong antioxidant activity with IC50 of 18.2106. Conclusions: The results of the research to determine the total phenol and total flavonoid content and antioxidant activity of cacao flower leaves showed that the total phenol content of the ethanol extract of cacao flower was 20.5803 ± 0.15 mgGAE/gram. Moreover, the total flavonoid content of the cacao flower ethanol extract showed a very strong antioxidant activity.

Telah dilakukan pemeriksaan parameter spesifik dan non spesifik dari seduhan dan Ekstrak Etanol Biji Kewer (Cassia ocidentalis Linn) sebelum dan sesudah roasting. Tanaman ini secara empiris dipergunakan sebagai tanaman herbal untuk pengobatan demam, batuk dan pasta dari daun ini dapat digunakan untuk penyakit kulit. Penelitian ini bertujuan untuk mengetahui parameter spesifik dan non spesifik dari air seduhan dan ekstrak etanol biji kewer sebelum dan sesudah roasting sesuai dengan FHI dan MMI. Karakterisasi simplisia dan analisis mikroskopik dilakukan terhadap simplisia yang telah dilakukan roasting dan sebelum roasting. Hasil uji parameter non spesisfik menunjukan bahwa nilai Kadar abu total sebelum roasting dan sesudah roasting sebesar 3,32% dan 3,72%. Kadar abu tidak larut asam sebesar 0,4% dan 0,41%. Kadar abu larut air 1,41 % dan 1,86%, kadar air 2%. Nilai untuk uji parameterik spesifik menunjukan kadar sari larut air sebelum roasting dan sesudah roasting 17,1 % dan 16,02%, kadar sari larut etanol 6,3% dan 5,59% sedangkan susut pengeringan sebesar 6,5% dan 4,5 %. Untuk hasil mikroskopis menunjukan adanya mesocarp, epidermis, mesofil, sklereida, jaringan lemak, trikoma, dan perikarp.

Antioksidan merupakan agen free radical scavengers artinya mampu bekerja mencegah dan memperbaiki kerusakan tubuh akibat radikal bebas. Senyawa antioksidan tersebut bisa kita peroleh salah satunya dari tanaman jambu Brazil batu (Psidium guineense L.) yaitu dengan mengoptimalkan kandungan senyawa pada ekstraksi dengan konsentrasi pelarut yang berbeda. Penelitian ini bertujuan untuk mengetahui konsentrasi pelarut etanol 96%, 70%, dan 50% yang memiliki aktivitas antioksidan yang paling optimal dari daun jambu biji brazil (Psidium guineense L.) dengan metode DPPH (2,2,-diphenyl-1- picrylhydrazyl). Metode Penelitian ini dimulai dari penyiapan simplisia, pengumpulan bahan, determinasi bahan, pembuatan simplisia, pemeriksaan karaktersitik simplisia, penapisan fitokimia, ekstraksi, uji aktivitas antioksidan dengan berbagai konsentrasi pelarut. Hasil penelitian dari penafisan fitokimia simplisia daun jambu brazil batu (Psidium guineense L.) teridentifikasi mengandung senyawa golongan alkaloid, flavonoid, saponin, tanin, kuinon, steroid/ triterpenoid. Kata kunci: Antioksidan, kepolaran, Psidium guineese L.