The practical application of waste recycling as feed supplement is potentially required by small-scale aquaculturist. Furthermore, some of the flour derived from waste sea urchin shells and in adopted in feed, including Deadema setosum were evaluated to estimate the effect on white shrimp Litopenaeus vannamei moulting. This research required the experimentation of four doses with triplicate supplementation (0, 2, 4, and 6 g 100 g-1 of feed) on shrimps four times daily (12% of body weight) for 35 days test period. The juvenile specimens, characterized by an initial weight of 1.61±0.11 g, were stocked at a density of 12 individuals in a 12 L aquarium. In addition, four compartments were created in each aquarium to facilitate progress observations. The proximate analysis results showed a 53.76±0.27% calcium content in the shell flour, which significantly increased (P0.05) after higher dose supplementation, in the sequential order 18.65±0.13%, 20.04±0.08%, 23.18±0.10%, and 25.04±0.11%. Moreover, the frequency and moulting interval with 4 g doses (16.59%±0.36% day-1 and 5.91±0.18 days-1) were significant (P0.05) and considered the best, compared to 0 g (10.48% ± 0.24% day-1 and 9.97±0.37 days-1), 2 g (13.49%±0.96% day-1 and 8.10±0.29 days-1), and 6 g (13.81%±0.24% day-1 and 7.90±0.06 days-1). In addition, the respective trend pattern for both parameters increased and decreased at 4 g and 6 g, correspondingly. The highest moulting intensity was also obtained with the 4 g doses, at a range of 4 to 6 times, while the lowest (0 g) varied from 3 to 4 times. These sea urchin shell flour was determined to have numerous practical applications as a feed supplement with proven ability to stimulate moulting in vannamei shrimp.Keywords:MoultingCalciumShellSea urchinFeed supplementationVannamei shrimp

Gracillaria changii is a high-value seaweed cultivated by the community on the seashore of Ujung Baji Village in Takalar Regency. This cultivation has become the local community's primary source of income, yet production and productivity are still inadequate. To address the issue, this technology dissemination aims to give cultivators with information and abilities in using the vertical method of cultivation to boost production and productivity aquaculture of G. changii . This dissemination used the Community Development (CD) method, which involved the community in every action, including planning, designing and assembling technology, implementation, and assessment. This dissemination was successful in increasing the Hati Mulia Cultivator Group's knowledge and capabilities in vertical seaweed cultivation. When compared to horizontal cultivation, this vertical cultivation technology can increase G. changii production and productivity by at least two to three times

CpG DNA merupakan imunostimulan DNA sintetik yang mengandung CpG motif, terdiri dari sekuen pendek satu atau lebih motif CpG dan berperan sebagai danger signal terhadap sistem kekebalan imun natural maupun bawaan. Penelitian bertujuan menganalisa kemampuan CpG DNA untuk meningkatkan ekspresi gen Interleukin 1β (IL1β) pada ikan kerapu macan agar dapat dijadikan sebagai imunostimulan. Analisis tingkat ekspresi gen dilakukan dengan cara mengekstrak RNA organ kepala ginjal yang diberi perlakuan CpG DNA dan perlakuan PBS sebagai kontrol.Sintesis DNA komplementer (cDNA) dilakukan menggunakan kit Ready To Go You Prime FirstStrand Beads (GE Healthcare) dengan teknik RT-PCR. Produk PCR selanjutnya dianalisa dengan UN SCAN IT berdasarkan ketebalan fragmen DNA. Tingkat ekspresi gen serta nilai luminescence photostimulated dari gen IL1β lebih tinggi dibandingkan dengan perlakuan PBS sebagai kontrol. Nilai luminescence photostimulated gen IL1β pada perlakuan CpG DNA yaitu 113684 sementarapada perlakuan PBS sebagai kontrol hanya sebesar 95610.

No abstract

In aquaculture, fish health problems are often encountered which can lead to failure in cultivation activities. One of the efforts to overcome the problem of disease in cultivation is done by providing immunostimulants. Sweet potato leaves (Ipomoea batatas) are plants that have a short harvest period, cheap and rich in vitamins and antioxidants so that it can be used as a source of immunstimulants. This research was conducted to determine the effect of adding sweet potato leaves (Ipomea batats) to tilapia (Oreochromis niloticus) feed on total leukocytes, phagocytic activity, hematocrit, and survival rate. The study was conducted by experimental method using a completely randomized design, with 4 treatment doses of sweet potato (Ipomoea batatas) used were 0%, 5%, 10% and 15%, which were formulated in fish feed and each treatment was repeated 3 times.. The results showed that giving 10% of sweet potato (Ipomea batatas) leaves in feed had a significant effect on the non-specific immune system of tilapia, which could increase the highest total leukocyte value by 6600 mm3 but did not have a significant effect on the phagocytosis index. 96.3%, 31% hematocrit with 93% survival.