Khuumii (throat or overtone singing) is a unique form of art derived from the nomadic population of Central Asia, which is a type of singing in which the singer manipulates the resonances (or formants) created as air travels from the lungs, past the vocal folds, and out of the lips to produce a melody. A total of 60 participants, aged 18-60 years (54 men and 6 women), were selected by non-random sampling method using cross-sectional study. X-ray, endoscopy, and sound research method were used in the study, and the composition of blood gas was analysed. X-ray examination determined the state during each different types of Khuumii; Shakhaa and Kharkhiraa. As the basic timbre of Shakhaa Khuumii went up progressively the larynx grew and the compression strength increased, while the basic timbre went down, and the larynx became lower. In the case of Kharkhiraa Khuumii, the larynx position was elevated to a relatively small extent compared to Shakhaa Khuumii and the distance between the sublingual bone and the larynx was large. The sublingual bone trunk lowered during Shakhaa Khuumii, while it was slightly elevated during Kharkhiraa Khuumii. The laryngeal endoscopy evaluated the movement of true and false vocal chords, glottal volume, movements of epiglottis and arytenoid cartilage, and mucosa. Furthermore, the sound frequency is 2-4 times higher than that of normal speech, and sound volume is 0.5-1 times higher. The blood gas composition test showed partial pressure (pO2), and saturation of oxygen (SaO2) decreased after performing Khuumii. In the case of Shakhaa and Kharkhiraa Khuumi, it is appropriate to divide Khuumii into two main types according to structural and functional changes in the organs involved.

Brown adipose tissues (BAT) have special active functions including thermogenesis, anti-obesity, and antidiabetic properties. Researchers have shown the immune roles of BAT in relation immune depression in deep hypothermia and lymphocyte accumulation in the spleen as detected in adipectomized rats. In this study we aimed to clarify immune functions of hibernating marmot BAT which continue to be used in pancreatitis treatment in Mongolia. In this study, 10 marmots were selected. The subject pool was limited license are needed for marmot use. Disengaged BAT was located between subcapularis and serratus anterior muscles. It was inserted into 96% of alcohol and 10% formalin for fixation. Histology examination was used with standard protocol of hematoxylin and eosin staining protocol. Fluorescenceactivated cell sorting method (Apogee Universal-50, England) was used to count the CD4+ and CD8+ T cells in the BAT of mice. The study selected BALB/c mode, 20 male mice 80-90 days-old. Marmots are not experimental animals therefore reagents of tissue cell count were limited. The BAT (marmot) histology observation showed connective tissue in the lobules reached by blood vessel branches and detected brown fat tissue surrounding follicle which included vessels with blood cells. Regarding tissue cell count results, the BAT detected a total of 24827/µL lymphocytes, which included 2056/µL (5.50%) CD4+ T cells and 991/µL (6.71%) CD8+ T cells, respectively. This study's results suggest the brown fat is may be an immune organ as shown by the lymphoid tissue follicle and lymphocyte subsets in the BAT.

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