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Globodera infestation was evaluated in soils of private farm plots in Chernihiv and Kyiv regions of Ukraine in 2017–2018. Soil samples were taken at 88 farms, 15.02 ha in total, in 11 settlements of 6 districts. The nematode abundance in the examined areas was graded as follows: less than 1000 eggs + larvae/100 cm3 — low rate of infestation; 1000–5000 eggs + larvae/100 cm3 — average rate; more than 5000 eggs + larvae/100 cm3 — high rate. 85.4 % of study area is infested with the golden potato cyst nematode. The mean abundance of pest was 3331 (15–23,237) eggs+larvae/100 cm3: including 8730 (91–21,486) eggs+larvae/100 cm3 in Kyiv Region and 1829.6 (15–23,237) eggs+larvae/100 cm3 in Chernihiv Region. The highest number of assessed plots per district (29) was in Koryukivsky District, and the mean abundance of G. rostochiensis (Rо-1) was 1549 (20–15,757) eggs + larvae/100 cm3e+l/100cm3. We also analyzed the results of golden nematode monitoring conducted by the State Service of Ukraine for food safety and consumer protection in 2018. According to those, the golden nematode is found in 18 regions of Ukraine. In most of those (14 regions), the pest infests rather small areas, less than 500 ha. Globodera is not recorded in the other 7 regions of Ukraine and Autonomous Republic of Crimea for now.

Rabbits are an important livestock animal species, which are used for their meat and fur. Nowadays they are also becoming more popular as pets. Furthermore, rabbits are commonly used in research, inter alia in immunological studies and for studying pathogenesis of human and animal diseases. The lymphoid tissue is abundant in the rabbit intestine and a lot of it is concentrated in Peyer's patches, the majority of which is located in the jejunum. Understanding of the rabbit Peyer's patches functions is essential for the prevention and treatment of their diseases. In order to enhance it, accurate knowledge of its lymphocyte population composition is needed. At present, the cellular composition of the rabbit gut-associated lymphoid tissue remains insufficiently studied. Therefore, the aim of our study was to establish the content and localization of lymphoid populations in the domestic rabbit jejunal Peyer’s patches.  We performed immunohistochemical studies using monoclonal antibodies CD3, CD10 and CD20 (DAKO, Denmark) to identify the distribution of lymphoid populations in jejunal Peyer's patches of a mature domestic rabbit. The results, obtained in our study, complement the existing data and determine the main lymphoid populations in the rabbit jejunal Peyer's patches. The presented data are fundamental for further studies of the rabbit gut-associated lymphoid tissue. We found that lymphoid nodules of jejunal Peyer's patches contains a predominant CD20+ cell population (B cells). It also contains many CD10+ cells (precursors of T cells and B cells), which are mostly concentrated in the germinal centers of lymphoid nodules. In the diffuse form of the lymphoid tissue the vast majority of cells are CD3+ cells (T cells)

The results of laboratory and field estimate of stability of newly created potato breeding material against Globodera rostochiensis (Ro1) are presented. During 2016—2018, 640 breeding samples sent by the leading breeding institutions of the state, namely: the Institute for Potato Research of the NAAS of Ukraine, the Polessky research department the Institute for Potato Research of the NAAS, CJSC NGO “Chernihivelitkartoplya” and the Institute of agriculture of the Carpathian region were tested in laboratory conditions. Among the potato material we studied, most of the samples belonged to the Polessky research department the Institute for Potato Research of the NAAS — 305 (47.7% of the total), where 172 samples (56.4%) of the sent hybrids of the first and second year of the testing were resistance. A higher percent of the resistance of the breeding material was in the potato material of the Institute for Potato Research of the NAAS, which was 68.0% (115 breeding numbers). In laboratory conditions, most of the samples — 57.7% showed resistance to golden potato cyst nematode (GPCN), 16.2% of the samples were weakly resistant and the rest samples — 26.1% of potato hybrids were classified as susceptible. During the field testing, 32 samples were tested, of which 28 were classified as resistance, 2 selective samples were weakly resistant and 2 were susceptible. The majority of resistance selection material on the high-invasive background showed high nematode cleanse ability and good yield, which exceeded control in 1.5—2 times. All resistant forms are a valuable material for introducing into the breeding process for the production of nematode resistant potato varieties, which will keep the crop in the infected areas and will have the ability to clear the soil from golden nematode potatoes.

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