Background Perfume is basically a cosmetic product applied to human body for an amusing scent or the feeling of freshness. A certain amount of perfume penetrates and remains attached to the protein of the skin when perfume is applied on the body. It evokes a surge of events in human immune system which results with allergic symptoms. Fragrance ingredients are leading cause that can be responsible for the occurrence of allergic contact dermatitis that is recently studied under cosmetic adverse reaction. Aim The aim of this review article was to define the allergies that are caused by fragrance ingredients. This review highlights the various aspects of perfume with respect to its manufacturing process, compositions, and fragrance ingredients identified as allergens and its present regulatory status. Method There area 175 frangrance ingredients that are used in perfumes cause allergic reaction. Several studies were conducted on the patients. The study was conducted on four fragrance markers in the baseline series: fragrance mix I (FM I), Myroxylon pereirae, fragrance mix II (FM II), and hydroxyisohexyl 3‐cyclohexene carboxaldehyde. Result Around 658 patients showed allergy due to fragrance ingredients when the patch test was performed. In other study, out of 1253 patients, 90% of the FM I and M. pereirae detected 90% of the cases. Conclusion Majority of the fragrance ingredients can cause allergic reactions and hence act as allergens and thus increase the risk of sensitization on activation. If any individual suffers from allergy or contact dermatitis on use of any perfume, he/she should be aware of it and should reduce or avoid its use to overcome such problems of hypersensitivity.

Objective: The present study deals with the development, validation and application of a simple, precise and accurate HPLC method for the determination of mycophenolate mofetil in pharmaceutical formulations and microemulsions. Methods: In this method, a simple isocratic mobile phase composition of methanol and water (75:25 v/v) pumped at 1 ml/minute flow rate through Phenomenex C18 column (dimension: 250 4.6 mm and 5 µm particle size) was used. Injection volume was 20 µl and analysis of mycophenolate mofetil was carried out at 250 nm. Results: The coefficient of regression was found to be 0.9996, indicating the linearity of the developed method within a range of 0.1 to 10 µg/ml. The limit of detection (LOD) and the limit of quantization (LOQ) were found to be 3.660ng/ml and 11.091ng/ml, respectively. The results showed that % deviation for change in compositions of the mobile phase, flow rate and temperature was within a range of-5.51 to 10.99%,-3.70 to 8.80% and-5.29 to 10.90%, respectively. The method seemed sensitive to change of temperature (±5 ○C) and methanol composition (±2%) as the results were at the boundary limit of 10% deviation. Conclusion: A simple, precise and accurate HPLC method for the determination of drug content from microemulsion has been developed and validated in accordance with ICH guidelines.

No abstract

No abstract