Brown rice is a type of rice can be grown on land that is less water (upland). Brown rice has anthocyanin as an antioxidant. The purpose of this study was to identify brown rice agronomics characters that can be used as selection criteria to obtain brown rice which high anthocyanin. The experiment was conducted at the greenhouse of Agriculture Faculty, UNS using completed randomized block design consisting of 5 local varieties namely Mandel Wonogiri, Segreng Gunung Kidul, Segreng Boyolali, Segreng Wonogiri 1, and Segreng Wonogiri 2. Analysis of the data using the model Singh and Chaudhary (1979) are displayed in the form of a matrix correlation. Anthocyanin assays using the differential pH test method. The results showed that there was no difference between the anthocyanin content of varieties, but there is a tendency Segreng Boyolali varieties containing anthocyanins and Mandel Wonogiri higher. In general, varieties tested showed different potential yield, except Mandel Wonogiri varieties have low yield. The character of the total number of tillers, panicle length, and number of panicles per hill showed a strong correlation coefficient with the results. Plant height, number of panicles per hill, and the weight of 1000 seeds showed a high correlation coefficient with anthocyanin content.

Abstract. Putri IIS, Yuniastuti E, Parjanto. 2022. The rambutan (Nephelium lappaceum L.) chromosomes. Biodiversitas 23: 2196-2202.  Rambutan (Nephelium lappaceum L.) is an annual plant with a wide distribution, it generates this plant to have diversity. The diversity is more often found in morphological characters, in this case, its genetics has not been discovered. The genetics of rambutan could also be indicated by its chromosome karyotype. This research was conducted using the squashing method. The genetic material used was four rambutan varieties which are: Ace, Binjai, Lebak Bulus, and Rapiah. For each variety, the study process will be repeated three times. The results of the study showed rambutan chromosome number in all varieties was 22 chromosomes (2n=22) with an average chromosome size of Ace was 1.777±0.363 µm, followed by Binjai was 1.477±0.392 µm, Lebak Bulus was 1.418±0.320 µm, and Rapiah was 1.443±0.315 µm, and the chromosome shape is metacentric. The karyotype formula obtained was 2n=2x=22=11m.

<p>Oyong production is still low due to the limited availability of quality seeds. The production can be increased by one of the plant breeding techniques, which is mutation technique. The technique of mutation by chemical mutagens is able to increase the genetic diversity ofplants allowing the breeding to select genotype of plants in accordance with the intended breeding objective. Therefore, research is required to be conducted by using colchicin as a chemical mutagen which causes polyploid, in  which  organism has  three sets  or  more chromosomes inside  its  cells. Meanwhile, the general nature of this polyploid plant is being sturdier; parts of the plantare bigger; so that later, its poor nature will be better as well as changing the potency of the result. Research using complete randomized block design (RBD) with one factor concentration of 0,1% (P1), 0,2% (P2), 0,3% (P3), 0,4% (P4), 0,5% ( P5), and 0,6% (P6).  Colchicines treatment can cause changes in  growth and  yield variables. Colchicines treatment with  a concentration of 0,3% showed positive changes to theweight of fruit, fruit diameter and length of the fruit. An increase in diversity on growth and yield components especially on colchicines treatment with 0,3%concentration.</p>

<p>A molecular marker of parent and offspring is used to find fast and accurate markers influenced by DNA isolation and amplification. This research aims to find the most suitable DNA isolation and DNA  amplification methods. This study used four DNA isolation methods; namely IM01, IM02, IM03, and IM04. DNA amplification used ten protocols (AP01, AP02, AP03, AP04, AP05, AP06, AP07, AP08, AP09, and AP010). The results of the research showed that the most suitable DNA isolation method for salak was  IM0, and the most suitable DNA amplification for salak was AP04 that produces the highest value of DNA bands.</p><p><strong> </strong></p><p>Keywords: DNA isolation; DNA amplification; hybrids.</p>

Abstract. Zulfahmi, Parjanto, Purwanto E, Yunus A. 2021. Genetic diversity and population structure of Eurycoma apiculata in Eastern Sumatra, Indonesia. Biodiversitas 22: 4431-4439. Information on genetic variation within and among populations of Eurycoma apiculata plants is important to develop strategies for their conservation, sustainable use, and genetic improvement. To date, no information on genetic variation within and among populations of the E. apiculata has been reported. This study aims to assess genetic diversity within and among populations of E. apiculata based on RAPD markers, and to determine populations to collect E. apiculata genetic material for conservation and breeding programs. Young leaves of E. apiculata were collected from six natural populations. Fifteen RAPD primers were used to assess the genetic diversity of each population. The data obtained were analyzed with POPGEN and Arlequin software. The amplification results of 15 selected primers produced 3-16 loci with all primers 100% polymorphic. At the species level, the mean allele per locus (Na), number of effective alleles (Ne), percentage of polymorphic loci (PPL), Nei’s gene diversity index (He) and Shannon information index (I) were 2.000, 1.244, 100%, 0.167, and 0.286, respectively. At the population level, the mean values for Na, Ne, PPL, He and I were 1.393, 1.312, 39.27%, 0.119, and 0.186, respectively. The highest value of gene diversity within population (He) was found in the Lingga-1 population and the lowest value was found in the Rumbio population. The value of genetic differentiation among populations (GST) of E. apiculata is 0.284, consistent with the results of the AMOVA analysis which found that genetic variation among populations was 23.14%, indicates that the genetic variation of E. apiculata was more stored within populations than among populations. The gene flow (Nm) value of E. apiculata was 1.259 migrants per generation among populations. The Nm value of this species was high category, and could inhibit genetic differentiation among populations. The clustering of E. apiculata population based on the UPGMA dendrogram and PCA was inconsistent with its geographic distribution, reflecting the possibility that genes migration occurred between islands in the past. The main finding of this study was the genetic variation of the E. apiculata mostly stored within the population. Therefore, the population with the highest genetic diversity is a priority for in-situ conservation, and collection of E. apiculata genetic material for ex-situ conservation and breeding programs should be carried out minimum from Lingga-1 and Pokomo populations.