Bacteriophage named as SAL-PG (Salmonella bacteriophage), specific to Salmonella pullorum and Salmonella gallinarum was isolated by an enrichment method directly from waste water near to poultry farm. For the isolation of phage 4.5 ml water sample, 0.5 ml of 10X Nutrient Broth and 0.5 ml of log phage bacteria were mixed well and incubated at 37 0 C for overnight followed by centrifugation at 10,000 rpm for 10 minutes. The presence of bacteriophage was observed by spot test over the bacterial lawn of and plaque assay. The host range of the isolated bacteriophage was determined by spot test using 12 different bacterial isolates. The phages were found to infect Salmonella pullorum, Salmonella gallinarum and Salmonella typhimurium and produced clear plaque on these Salmonella serovars. The bacteriophage was able to survive in wide range of pH between 2 to 9 and resistant at 60°C for 1 hour. More than 50% phages were readily adsorbed to the host bacteria. The restriction enzyme analysis showed that the phage DNA possess restriction site for TaqαI, HindIII and BstYI; but no restriction site for XhoI and BstEII as these two enzymes failed to digest the phage DNA. The characterizations of the bacteriophage would be helpful in establishing a basis for adopting the application of the most effective bacteriophage treatment to control these poultry bacteria.

An experiment was conducted in a covered

No abstract

The study was conducted in 16 coastal district viz. Satkhira, Khulna, Mongla, Khepupara, Barishal, Bhola, Patuakhali, Hatiya, Chandpur, Feni, Swandip, Sitakunda, Chittagong, Kutubdia, Cox’s Bazar and Teknaf of Bangladesh during the period of 1975-2014 to investigate the variation of sea level pressure (SLP) and rainfall. SLP data of 40 years (1975-2014) have been analyzed in this study. The SLP was increasing slightly to the month of September and then a rapid increasing was observed from the month of October to December. Of all the stations, in the month of June and July the lowest SLP was observed in the Khulna region and highest in Teknaf. In the month of December and January the highest and lowest SLP was found in the Month of January. As the fluctuation of SLP was high in the summer season the possibility of cyclone occurrence also rise in this season. The average rainfall is lowest in the station Sitakunda and highest in Swandip. The average rainfall of all district divided the stations into two parts. The rainfall of Barishal, Bhola, Chandpur, Mongla, Sitakunda and Khulna is lower the average of all district. This shows a great instability of weather in these regions. Rest of the stations has the higher average rainfall than the average rainfall of all stations.Progressive Agriculture 27 (3): 285-295, 2016