Although total blood loss and postoperative hemoglobin drop are significant greater in EACA groups, there is no significant difference between TXA and EACA groups in terms of transfusion rates. Based on the current evidence available, higher quality RCTs are still required for further research.

Osteosarcoma (OS) is an aggressive malignant neoplasm that arises from primitively transformed cells of mesenchymal origin, and that exhibits osteoblastic differentiation and produces malignant osteoid. MicroRNAs (miRNAs) have been widely reported to have important regulatory roles in various human tumors, including OS. However, the potential mechanism of miR-29 in OS remains largely unknown. miR-29 was highly expressed in OS and overexpression of miR-29 promoted OS cell proliferation, as well as proliferating cell nuclear antigen (PCNA) expression and migration, whereas lower expression of miR-29 inhibited OS cell proliferation, PCNA expression and migration. In the present study, a dual-luciferase reporter system supporting phosphatase and tensin homolog (PTEN) was a target of miR-29 and its expression was inhibited by miR-29 mimic, but increased by miR-29 inhibitor. Overexpression of PTEN inhibited OS cell proliferation and migration and it could attenuate miR-29 promotion effect on OS progression. Overall, the results revealed that miR-29, as a tumor promoter, is involved in OS progression and metastasis by targeting PTEN, indicating that the miR-29/PTEN pathway is a potential therapeutic target for the treatment of OS.

Background: The publication citation number incarnates the impact of the article in a special research field. Bibliometric analysis regarding the top cited publications has been extensively used in orthopedics and its subspecialties. Whereas， to our knowledge, no bibliometric analysis in clubfoot research area has been made. We aimed to identify and analyze the characteristics of the top 50 cited publications on clubfoot. Methods: Publications on clubfoot from 1945 to 2022 were retrieved from the Web of Science (WoS) core collection database. Different aspects of top 50 cited articles, including titles, journals, authors, decades, citation number, institutions, countries, topics, study types, evidence levels, impact factor, impact index were recorded and analyzed.Results: The top 50 cited papers were published from 1962 to 2011 with total citation number ranged from 380 to 63. The 2000s was the most productive decade. Fifteen journals constituted of top 50 publications. United States hold the majority of originated countries, followed by United Kingdom and France. Outcome was the most popular topic discussed. Level III was the most common evidence level in top 50 cited articles. Clinical studies constituted the most of publications.Conclusions: This study may present valuable insights into the development of clubfoot research and provide help for researchers who want to obtain the important information on the classic publications.