Objective Osteoarthritis (OA) is the most prevalent joint disease characterized by the degeneration of articular cartilage and the remodeling of its underlying bones, resulting in pain and loss of function in the knees and hips. As far as we know, no curative treatments are available except for the joint replacement. The precise molecular mechanisms which are involved in the degradation of cartilage matrix and development of osteoarthritis are still unclear. Design By analyzing RNA-seq data, we found the molecular changes at the transcriptome level such as alternative splicing, gene expression, and molecular pathways in OA knees cartilage. Results Expression analysis have identified 457 differential expressed genes including 266 up-regulated genes such as TNFSF15, ST6GALNAC5, TGFBI, ASPM, and TYM, and 191 down-regulated genes such as ADM, JUN, IRE2, PIGA, and MAFF. Gene set enrichment analysis (GSEA) analysis identified down-regulated pathways related to translation, transcription, immunity, PI3K/AKT, and circadian as well as disturbed pathways related to extracellular matrix and collagen. Splicing analysis identified 442 differential alternative splicing events within 284 genes in osteoarthritis, including genes involved in extracellular matrix (ECM) and alternative splicing, and TIA1 was identified as a key regulator of these splicing events. Conclusions These findings provide insights into disease etiology, and offer favorable information to support the development of more effective interventions in response to the global clinical challenge of osteoarthritis.

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Abstract:In this paper, we mainly described the reversible self-assembly of a backbone-thermoresponsive, long-chain, hyperbranched poly(N-isopropyl acrylamide) (LCHBPNIPAM) in aqueous solution. Here, we revealed a reversible self-assembly behavior of LCHBPNIPAM aqueous solution derived from temperature. By controlling the temperature of LCHBPNIPAM aqueous solution, we tune the morphology of the LCHBPNIPAM self-assemblies. When the solution temperature increased from the room temperature to the lower critical solution temperature of PNIPAM segments, LCHBPNIPAM self-assembled from multi-compartment vesicles into solid micelles. The morphology of LCHBPNIPAM self-assemblies changed from solid micelles to multi-compartment vesicles again when the temperature decreased back to the room temperature. The size presented, at first, an increase, and then a decrease, tendency in the heating-cooling process. The above thermally-triggered self-assembly behavior of LCHBPNIPAM aqueous solution was investigated by dynamic/static light scattering, transmission electron microscopy, atomic force microscopy, fluorescence spectroscopy, 1 H nuclear magnetic resonance in D 2 O, and attenuated total reflectance Fourier transform infrared spectroscopy. These results indicated that LCHBPNIPAM aqueous solution presents a reversible self-assembly process. The controlled release behaviors of doxorubicin from the vesicles and micelles formed by LCHBPNIPAM further proved the feasibility of these self-assemblies as the stimulus-responsive drug delivery system.

PbS nanocrystals with octahedron shape are synthesized by a low temperature approach with presence of cation/anion surfactants in aqueous solution. CdS quantum dot sensitized solar cells (QDSSCs) based on these novel octahedron shaped PbS counter electrodes (CEs) are fabricated for the first time, achieving PCE of 1.54%. It is observed that the octahedron shaped PbS CE shows higher electrocatalytic activity compared with the Pt CE, indicating that the PbS nanocrystals with a uniformly distributed size is a superior candidate as CEs for QDSSCs application. This work may provide new route for preparing PbS from aqueous solvent medium through a simple synthesis route.

The late Eocene succession of the Dongying Depression forms a highly productive hydrocarbon source. However, due to lack of an unambiguous fine chronostratigraphic framework for the late Eocene stratigraphy, it is challenging to understand the paleolake’s evolution and the driven mechanism of lake-level variation, a limitation which hinders hydrocarbon exploration. In this work, high-resolution gamma-ray logging data were analyzed to carry out the cyclostratigraphic analysis of the third member (Es3) of the Shahejie Formation in the Dongying Depression. Significant 405-kyr eccentricity cycles were recognized based on time series analysis and statistical modeling of estimated sedimentation rates. We abstracted ~57 m cycles of the GR data in the Es3 member, which were comparable with the long eccentricity cycles (~405-kyr) of the La2004 astronomical solution, yielding a 6.43 Myr long astronomical time scale (ATS) for the whole Es3 member. The calibrated astronomical age of the third/fourth member of the Shahejie Formation boundary (41.21 Ma) was adopted as an anchor point for tuning our astrochronology, which provided an absolute ATS ranging from 34.78 ± 0.42 Ma to 41.21 ± 0.42 Ma in Es3. According to the ATS, sedimentary noise modeling for the reconstruction of lake-level changes was performed through the late Eocene Es3. The lake-level changes obtained based on sedimentary noise modeling and spectrum analysis reveal significant ~1.2 Myr cycles consistent with global sea level variations which were related to astronomical forcing. Potential driven mechanisms of marine incursion and/or groundwater table modulation were linked to explain the co-variation of global sea level changes and regional lake level changes. Our results suggest global sea level fluctuations may have played an important role in driving the hydroclimate and paleolake evolution of the late Eocene Dongying Depression.