The strong regulation of plant carbon allocation and growth by trehalose metabolism is important for our understanding of the mechanisms that determine growth and yield, with obvious applications in crop improvement. To gain further insight on the growth arrest by trehalose feeding, we first established that starch-deficient seedlings of the plastidic phosphoglucomutase1 mutant were similarly affected as the wild type on trehalose. Starch accumulation in the source cotyledons, therefore, did not cause starvation and consequent growth arrest in the growing zones. We then screened the FOX collection of Arabidopsis (Arabidopsis thaliana) expressing full-length cDNAs for seedling resistance to 100 mM trehalose. Three independent transgenic lines were identified with dominant segregation of the trehalose resistance trait that overexpress the bZIP11 (for basic region/ leucine zipper motif) transcription factor. The resistance of these lines to trehalose could not be explained simply through enhanced trehalase activity or through inhibition of bZIP11 translation. Instead, trehalose-6-phosphate (T6P) accumulation was much increased in bZIP11-overexpressing lines, suggesting that these lines may be insensitive to the effects of T6P. T6P is known to inhibit the central stress-integrating kinase SnRK1 (KIN10) activity. We confirmed that this holds true in extracts from seedlings grown on trehalose, then showed that two independent transgenic lines overexpressing KIN10 were insensitive to trehalose. Moreover, the expression of marker genes known to be jointly controlled by SnRK1 activity and bZIP11 was consistent with low SnRK1 or bZIP11 activity in seedlings on trehalose. These results reveal an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway involving T6P, SnRK1, and bZIP11.

The objective of this research was to test antibacterial activity ethanol and ethyl acetate of lime peel (Citrus aurantifolia Swingle) extract by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) against the growth of clinical isolate bacteria. The type of this study used laboratory experimental using sensitivity test, dilution of tube and spreading plate. The experimental design used Complete Random Design using three repetitions on three concentrations i.g 25, 50, and 75%. Data was analyzed by qualitative with measuring inhibit diameter zone bacterial and qualitative using Two Way Anova followed by BNT with α= 5%. The statistical analysis showed that the solvent factor and concentration factor was significantly against growing bacterial and there was an interaction affect of both factors on S. aureus growth. The result presented that the ethanol and ethyl acetate of lime peel extract strongly inhibited Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa; and midly inhibited the growth of Klebsiella pneumoniae. The MIC ethanol and ethyl acetate of lime peel extract against S. aureus, S. epidermidis and P. aeruginosa was 25% and K. pneumoniae was 50%. The MBC showed that ethanol and ethyl acetate of lime peel  extract killed bacteria P. aeruginosa at 15%, S. aureus, S. epidermidis at 20% and K. pneumoniae at 25%. Taken together, it can be concluded that ethyl acetate of lime extract more effective than that of ethanol extract was to inhibit and killed clinical isolate bacterial.Keywords: lime peel (Citrus aurantifolia Swingle), antibacterial activity, clinical isolate bacteria, MIC and MBC.

The tropical climate in Indonesia supports the presence of many types of plants that have the potential to produce abundant vegetables and fruit, however the presence of fruit flies is an obstacle to fruit and vegetable productivity. Lemongrass and basil have been studied to contain several compounds that have the potential to control fruit fly attacks. This study aims to determine the effect of the ethanol extract of lemongrass pseudo stem (Cymbopogon citrates) and basil (Ocimum sanctum) leaves on the catch of Bactrocera fruit flies and to determine the concentration of the extract that has the highest number of catches. The method used in sampling was factorial randomized block design (RBD). The ethanol extract of the pseudo-lemongrass, the extract of basil leaves or the mixture of both extract (1:1) that was given at concentrations of 15%, 30% and 45% was dropped onto a piece of cotton and for then be put within a trap before placing on the tree. The cathes obtained from each extract at each concentration were observed if they show a significant difference in the number of fruit fly. Data were tested using analysis of variance (F test) at α = 0.05, followed by the Honest Significant Difference test (HSD). The results showed that the catch obtained from ethanol extract of lemongrass pseudo stem or from basil leaf ectract as well as  from their mixture had a significantly higher number of Bactrocera fruit flies than without using these extracts. The difference in extract concentration did not cause a difference in the ability to attract fruit flies. The conclusion of this study is that there is an effect of the  pseudo stem ethanol extract of lemongrass or basil leaf ectract as well as their mixture on the catch of Bactrocera fruit flies and these extract are recommended as non-synthetic pesticidesalternatives.