A mussel mimetic transdermal patch
was prepared using bacterial
cellulose (BC), a green resource derived from Glucanoacetobacter
xylinus (MTCC7795). To impart the mussel mimetic property,
dopamine (DOPA), a catechol-containing compound, was used to modify
the isolated BC via an amidation reaction between the carboxylated
BC and DOPA, and the end product was successively characterized by 1H NMR and FTIR analysis. The free hydroxyl group of the DOPA
moiety of DOPA-modified BC (BC-DOPA) was utilized to prepare BC-DOPA/rGO/Ag
NPs, a composite film incorporating reduced graphene oxide/silver
nanoparticles (rGO/Ag NPs). The antimicrobial action of the prepared
film was determined against both Gram-positive (Staphylococcus
aureus and Lysinibacillus fusiformis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria.
The bactericidal property of the composite film was determined using
the zone of inhibition (ZOI) method and live–dead assay (DAPI–PI
analysis). The morphological transformation of bacteria upon the application
of the composite film was observed through SEM analysis. The cell
compatibility of the composite film over the NIH 3T3 fibroblast cell
line was assessed through an XTT assay. The in vitro wound-healing
assays over the NIH 3T3 cell line and A549 human lung epithelial cell
line reveal that the presence of rGO and Ag NPs in the composite film
accelerates the wound-healing process.
Green chemistry C-Dot-based ‘turn-on’ and ‘turn-off’ fluorescence sensor for pesticides [imidacloprid (LOD ∼ 0.013 μM) and tetradifon (LOD ∼ 0.04 μM)] in aqueous solution.
A layer-by-layer (L-B-L) bacterial cellulose (BC)-based transdermal patch has been prepared via a Schiff base reaction. The L-B-L assembly consisting of covalently cross-linked ethylene diamine-modified carboxymethylated BC isolated from the Glucanoacetobacter xylinus (MTCC7795) bacterial strain and aldehydemodified pectin formed via a Schiff base reaction. The presence of the imine bond assists the self-healing process after being scratched in the presence of a pH 7.4 buffer solution monitored via optical microscopy, atomic force microscopy, and tensile strength analyses. The formation of the L-B-L assembly was confirmed using field-emission scanning electron microscopy (FESEM) analysis. Simultaneously, water swelling and deswelling studies were carried out to test its water retention efficiency. The presence of silver nanoparticles (AgNPs) has been confirmed by ultraviolet−visible spectroscopy and FESEM analyses. The antimicrobial activity of the AgNPs-incorporated transdermal patch has been examined over Staphylococcus aureus and Escherichia coli using the zone of inhibition method. Additionally, the cell viability assay was performed using the fluorescent dyes 4′,6-diamidino-2-phenylindole and propidium iodide. The AgNPs in the L-B-L assembly showed antimicrobial property against both types of bacteria. The cytotoxicity and wound healing property of the patch system have been studied over NIH 3T3 fibroblast and A549 epithelial cell lines. The L-B-L film also influenced the wound healing process of these two cell lines.
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