BackgroundDysregulation of miR-9 is a common feature of many types of cancers, including oral squamous cell carcinoma (OSCC). However, whether the expression level of serum miR-9 is changed in patients with OSCC remains unknown.Material/MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine the expression level of serum miR-9 in OSCC patients, oral leukoplakia (OLK) patients, and healthy volunteers, then we evaluated the association between serum miR-9 expression level and clinical outcome of OSCC patients.ResultsThe expression level of serum miR-9 was significantly downregulated in patients with OSCC or OLK in comparison with healthy controls (P<0.01). Serum miR-9 expression level was associated with various clinicopathological parameters, including T stage (P=0.013), lymph node metastasis (P=0.002), and TNM stage (P=0.007). In addition, the OSCC patients in the low serum miR-9 expression group had poorer overall survival rate (P=0.022) and disease-free survival rate (P=0.004) compared with those in the high serum miR-9 expression group. Multivariate analysis showed that serum miR-9 was an independent prognostic factor for OSCC.ConclusionsSerum miR-9 was downregulated in patients with OSCC and patients with OLK. In addition, low serum miR-9 was correlated with poor prognosis of OSCC, indicating miR-9 might play a tumor suppressive role in OSCC and can serve as a promising biomarker for this deadly disease.

Synthesis-modification-doping-integration: one-step synthesis of boronic acid-decorated carbon nanodots for glycoprotein sensing.

In the present study, RNA interference (RNAi) was used to investigate the effect of vascular cell adhesion molecule 1 (VCAM1) silencing on the proliferation of human oral squamous carcinoma HN12 cells. HN12 cells were divided into three groups: The untreated blank control cell group (CK), the negative control group transfected with non-homologous vector (NC) and the positive group transfected with the target sequence VCAM1 small hairpin RNA (KD). Reverse-transcription polymerase chain reaction and western blot analysis were used to examine the effects of VCAM1-knockdown on the mRNA expression of VCAM1 and subsequent protein expression. Furthermore, the HN12 cell growth inhibition rate was detected using the cell counting kit-8 method. The VCAM1-targeted lentiviral vector RNAi significantly inhibited VCAM1 mRNA, and subsequent protein, expression. Compared with the NC group, the VCAM1 gene knockdown efficiency was ~85%, while the expression level of VCAM1 protein was reduced by ~74% in KD group cells. In addition, cell growth was significantly inhibited in the KD group, with a growth inhibition rate of ~34%. Therefore, this targeted lentiviral vector RNAi effectively inhibited VCAM1 gene, and subsequent protein, expression, as well as the proliferation of oral squamous carcinoma cells. These results may provide an experimental reference for the diagnosis and treatment of oral squamous cell carcinoma.

Metal-organic frameworks (MOFs) are promising nanomaterials with unprecedented capacity to store small molecules. Titanium cooperated with NH 2-UiO-66 were prepared via post synthesis modifications approach and their structures and properties were characterized by X-raydiffraction,Fourier transform infrared spectroscopy, thermogravimetric analysis, nitrogen adsorption isotherms, and scanning electron microscopy. The resultant titanium cooperated with NH 2-UiO-66 showed excellent performance for CO 2 adsorption via the formation of oxo-bridged hetero-Zr-Ti clusters. This result clearly demonstrates that the smaller Ti ions exchanged with the Zr ions of NH 2-UiO-66 may decrease the pore sizes within the framework to be closer to the ideal pore sizes for CO 2 adsorption.

Background: Antenatal depression (AD) has adverse effects on mothers and children. While pregnant women in China are given less attention in terms of their mental health, in contrast, AD has resulted in increased interest at the international level. Methods: This study reviewed 1881 studies on AD from the Web of Science (WOS) and China National Knowledge Infrastructure (CNKI), using the bibliometric method in CiteSpace, to systematically analyze the research status, research hotspot, and potential trends of research on AD in China and abroad. Results: The results showed that: (1) There are 511 papers from the United States, followed by 210 from England, 136 from Australia, and 116 from Canada. Furthermore, articles from these four countries have the highest influence. And that the quantity and influence of papers published in China are relatively low; (2) Institution with the most publications is located in England, and those with the most influence are located in Australia and the United States; there are few Chinese institutions that publish on AD; (3) Literature on WOS clustered 7 hot topics, while documents on CNKI clustered 6, with similarities and differences; (4) With the passage of time, the researches of AD on CNKI gradually focused on the investigation and intervention of specific groups, while researches on WOS tend to consistently explore the biological and psychological mechanism and variety of intervention measures. Conclusions: It is the goal of China's research to further explore the mechanisms and influencing factors of AD in order to better implement diversified interventions and improve the quality of life for mothers with AD and their offspring.