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SUMMARYBlack queen-cell virus was isolated from dead prepupae and pupae of queens and workers of Apis mellifera found locally in the field. Kashmir bee virus was isolated from individuals of Apis mellifera that had died in the laboratory after they had been inoculated with some preparations from Apis cerana. Both viruses have isometric particles about 3o nm in diameter, contain RNA, and are unrelated to each other or to any known bee virus. Black queen-cell virus particles sediment at I5IS and have a buoyant density in CsCI of 1.345 g/ml; Kashmir bee virus particles sediment at 17I to I73S and have a buoyant density in CsCI of 1-371 g/ml. Serological evidence indicates that black queen-cell virus is common in Britain and occurs in the U.S.A. The studies involved acute bee-paralysis and sacbrood viruses and led to re-determination of the buoyant densities of these as 1.38o and 1.358 g/ml respectively.

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SUMMARYArkansas bee virus, bee virus X and slow paralysis virus, isolated from adult honey bees, have isometric particles, contain RNA and are serologically unrelated to each other or to the other known bee viruses. Arkansas bee virus particles are 3o nm in diam. sediment at 128 S, have a buoyant density in CsC1 of 1.37 g/ml and kill bees injected with them in about 3 weeks. Bee virus X particles are 35 nm in diam., sediment at I87S, have a buoyant density of I '36 g/ml, multiply when fed to young bees kept at 3o °C but not at 35 °C nor when injected, and have not by themselves been associated with symptoms or mortality, although they killed bees when injected in combination with sacbrood virus. Slow paralysis virus particles are 3o nm in diam., sediment at about I76S, have a buoyant density of 1'35 g/ml and kill bees injected with them in about I2 days.