This study aims to find bioactive metabolites from fungus, which was isolated from marine sponge Haliclona fascigera. The fungus was identified molecularly as C. geniculatus WR12. The isolation of the bioactive compound was achieved by column chromatography with step gradient polarity (SGP) method and purification by recrystallization. The structure was determined based on spectroscopic analyses (UV, IR, HR-MS, 1 H and 13 C NMR). The antibacterial activity of the compound was tested against Methicillin-Resistant Staphylococcus Aureus (MRSA) by using the agar diffusion method. The Cytotoxicity of the compound was tested on the cancer cell lines by using the microculture tetrazolium (MTT) assay. The results of this study were obtained a pure compound (1). The compound was identified as radicinin based on 1D and 2D NMR and HRESIMS data. This compound showed cytotoxic activity with IC 50 values of 60.68, 30.89, and 87.89 µg/mL against WiDr, T47D, and Hela cell lines, respectively, but not toxic against Vero cell (IC 50 value of 607.31 µg/mL). Radicinin showed higher cytotoxic activity against T47D cells (IC 50 = 25.01 ppm) compared with doxorubicin (IC 50 = 33.49 ppm). The result of the antibacterial activity of radicinin showed the minimum inhibitory concentration (MIC) of 125 µg/disc against MRSA. Based on these results, it can be concluded that radicinin could be considered as a potential candidate for future anticancer and anti-MRSA drug.

Breast cancer is a non-communicable disease that is the main killer for most women in the world. One of ten women with breast cancer will not survive more than five years after diagnosis. Breast cancer cases in Indonesia have been increased annually. As a nucleotide-metabolizing enzyme, NUDT5 (NUDIX hydrolase) catalyzes ADP to ATP nuclear that is used for cell proliferation. NUDT5 is a regulator of tumor driver in breast cancer proliferation. ATP nuclear synthesis can be blocked by inhibiting the NUDT5. The study of potential therapeutic inhibitors for NUDT5 was increased because the current treatment for breast cancer has side effects. Vanilla planifolia is a commercial plant from Orchidaceae which contains secondary metabolites and medicinal potential. This research aims to examine the metabolite of V. planifolia as the inhibitor of NUDT5 activity using molecular docking studies. V. planifolia metabolite and NUDT5 (5nwh) 3D structure were downloaded from the database (RSCB, Pubchem, and Dr. Duke), then the prediction of ADME (absorption, distribution, metabolism, and excretion) and potential bioactivity of the metabolite was analyzed by online structure-based prediction (SwissADME and Way2drug). After that, molecular docking was performed by AutoDock Vina. Three metabolites with the highest binding affinity scores were analyzed by visualization software (Discovery Studio). The result shows that Naphthalene, Caproic acid, Trimethylacetophenone, and Methyleicosane have the highest binding affinity. This result indicated that Naphthalene, Caproic acid, Trimethylacetophenone have the potential effect to inhibit NUDT5 enzyme thus decrease the potential of breast cancer.

Begomovirus infects various species of the Solanaceae family and reduces crop productivity. It is important to note that Bemisia tabaci is a vector that contributes to increasing infection. Therefore, this study aims to identify and modify DNA extraction methods, as well as detect the presence of Begomovirus in whitefly and eggplants (Solanum melongena L.) plantations. S. melongena leaves showing infection symptoms were collected from eggplant cultivation in Rejodani,

Abstract Orchidology is part of the branch of botany, it deals with a large family of monocotyledonous plants, Orchidaceae. Orchids have been used as traditional herbal medicines in Indonesia which are believed to be antimicrobial and anti-cancer. Orchids are able to produce secondary metabolites as their protective agent due to the extreme environment. Orchids have great diversity and potential to be the object of research, not only in experimental studies but also in a computational studies like in silico. Nowadays, molecular or metabolite data are available on the official-standard website as an international database. This study was conducted by analyzing web-based data to provide information about the potential of orchids that have been trusted as herbal medicines. In this study, secondary metabolite from Vanilla spp., Dendrobium spp., and Vanda spp., were selected in the existing literature as antimicrobial and anti-cancer drugs. Secondary metabolites obtained from the KNApSAck-3D core system and Phytochemical and Prediction of Substance Activity Spectrum (PASS) were performed to determine the potential anti-cancer and antimicrobial activity. Based on in silico analysis through PASS online, the secondary metabolites of orchids that have potential as antimicrobials in this study were Dendroside E and Dendromonilised D from Dendrobium also Parviflorin, Licoisoflavone and Luteine ​​extracted from Vanda spp. The secondary metabolite of Vanilla planifolia which has potential as anti-cancer for further research is 4-Hydroxybenzoic acid as a Chlordecone reductase inhibitor extracted from shoots.   Keywords: orchid, antimicrobial, anticancer, secondary metabolite, PASS online