The objective of present research work was to develop methotrexate loaded chitosan anchored shell nanoparticles for drug delivery in breast cancer. Chitosan nanoparticles (CS-NPs) were synthesized by ionic gelation of chitosan using sodium tripolyphosphate (STPP). The optimized nanoparticles were characterized for particle size and polydispersity index (PDI) revealed particle size were found to be between 57.08 nm to169.5 nm and PDI 0.252 to 0.639 respectively. The results signpost that stirring speed during ionic gelation reaction was also decisive parameters for the size of the nanoparticles obtained. Further characterization involved to show polymer-drug interaction was FTIR and DSC. This paper grants a revision of the physical factors elaborate in attaining nanoparticles in order to regulate the particle size of polymeric nanoparticles made from chitosan, without any surplus chemical treatment. Keywords: Breast cancer, Nanoparticles, Chitosan, Methotrexate

Cancer is one among the leading causes of death worldwide. Cancer cells proliferate at much faster rate than the normal cells. The available traditional cancer chemotherapy is not essentially selective as it depends on the kinetics of the cell growth. 1 Breast cancer is common lethal cause of malignancy among women around all the countries. Early detection of breast cancer facilitates the diagnosis and treatment prior to metastasis. Despite remarkable development in new medicine findings and therapies for carcinoma during previous decades, no significant treatment methods are accessible for canceraffected people with invasive and metastatic carcinoma. During this stage, the patients have less response to cancer therapy thanks to recurrence properties of cancer.

Cancer has become a solemn threat to the life of human beings universally. Various strategies are available to steadfastness cancer; however they are not so effective owed to their serious side effects, noxious effect to healthy cells and non- specificity to cancer cells targeting. To tenacity above facts we try to deed inherent characters of cancer cells. HA was used as a targeting agent for drug delivery to breast cancer cells. In this work nanoparticles were equipped using chitosan and sodium tripolyphosphate encapsulating methotrexate. Methotrexate (Mtx) a folic acid antagonist that inhibits dihydrofolatereductase (DHFR) and blocks conversion of dihydrofolic acid (DHFA) to tetrahydrofolic acid (THFA) of the cell cycle. Chitosan anchored nanoparticles were prepared by ionotropic gelation method by means of sodium tripolyphosphate and evalauted for in-vitro drug release study with dialysis membrane. Result depicts that drug releases from chitosan nanoparticles in sustained manner over a prolonged episode of time from the NPs as the medium acidity enhanced at the target site, not in plasma. In conclusion, chitosan anchored nanoparticles of MTX could be well thought-out as probable candidate for drug delivery in the treatment of breast cancer. Keywords: Breast cancer, Methotrexate, Chitosan, TPP and Nanoparticles.