RESUMO O presente trabalho teve como objetivo avaliar as condições higiênicas e conservatórias de maioneses caseiras comercializadas no município de Juazeiro do Norte-CE. Através de análises microbiológicas, as amostras adquiridas foram inoculadas no meio de enriquecimento Brain Heart Infusion (BHI), onde permaneceram por 24 horas na estufa, em seguida as amostras inoculadas foram semeadas nos meios de cultura Agar Manitol Salgado, Ágar SS (Salmonella-Shiguella) e Agar Methylene Blue Eosin (EMB), após 24 horas foram feitas as identificações bacterianas através do método de coloração de Gram e provas bioquímicas para identificação das espécies. Foram coletadas 36 amostras, 80,6% dessas apresentaram contaminação bacteriana, dentre as bactérias encontradas 89,6% foram Stapylococcus aureus, 6,9% foram Escherichia coli e 3,5% foram Salmonella sp. Os resultados obtidos por meio dessa pesquisa podem estar relacionados com o armazenamento e contaminação por higienização inadequada.
The objective of this work was to evaluate the phytochemical composition and the antibacterial and antibiotic-modulating activities of the essential oils of Eugenia brasiliensis Lam (OEEb) and Piper mosenii C. DC (OEPm) singly or in association with blue LED (Light-emitting diode) light. The antibacterial and antibiotic-modulatory activities of the essential oils on the activity of aminoglycosides were evaluated to determine the minimum inhibitory concentration (MIC, μg/mL) in the presence or absence of exposure to blue LED light. The chemical analysis showed α-pinene and bicyclogermacrene as major constituents of OEPm, whereas α-muurolol was the main compound of OEEb. Both OEEb and OEPm showed MIC ≥ 512 μg/mL against the strains under study. However, the association of these oils with the blue LED light enhanced the action of the aminoglycosides amikacin and gentamicin. In conclusion, the association of aminoglycosides with the blue LED light and essential oils was effective against resistant bacteria.
Background: Pathogenic microorganisms are causing increasing cases of mortality and morbidity, along with alarming rates of ineffectiveness as a result of acquired antimicrobial resistance. Bi2WO6 showed good potential to be used as an antibacterial substance when exposed to visible light. This study demonstrates for the first time the dimension-dependent antibacterial activity of layered Bi2WO6 nanosheets. Materials and methods: The synthesized layered Bi2WO6 nanosheets were prepared by the hydrothermal method and characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and Raman and Fourier transform infrared spectroscopy (FTIR). Antibacterial and antibiotic-modulation activities were performed in triplicate by the microdilution method associated with visible light irradiation (LEDs). Results: Bi2WO6 nanosheets were effective against all types of bacteria tested, with MIC values of 256 μg/mL against Escherichia coli standard and resistant strains, and 256 μg/mL and 32 μg/mL against Staphylococcus aureus standard and resistant strains, respectively. Two-dimensional (2D) Bi2WO6 nanosheets showed antibacterial efficiency against both strains studied without the presence of light. Conclusions: Layered Bi2WO6 nanosheets revealed dimension-dependent antibacterial activity of the Bi2WO6 system.
The present study aimed to investigate the antibiotic-enhancing activity of the essential oils obtained from the leaves of Eugenia brasiliensis Lam (EOEb) and Piper mosenii C. DC. (EOPm) against multiresistant strains of Escherichia coli 06 and Staphylococcus aureus 10. The essential oils were obtained by hydrodistillation, and the minimum inhibitory concentrations (MIC) were determined through the broth microdilution method. The EOEb enhanced the activities of norfloxacin and erythromycin against Staphylococcus aureus and Escherichia coli, respectively. At the same conditions, the EOPm was found to potentiate the effects of gentamicin and erythromycin against E. coli. Of note, this is the first study to date that has reported the ability of these extracts to enhance the antibacterial activity of conventional antibiotics.
Este estudo buscou analisar a qualidade bacteriológica da água do rio Salgadinho no município de Juazeiro do Norte, Ceará, por meio da pesquisa de coliformes totais e termotolerantes. As coletas foram feitas onde é evidente o despejo de resíduos domésticos e industriais, no período de agosto a setembro de 2016 com dez dias de intervalo entre cada uma. O procedimento de análise foi realizado em triplicata, utilizando o método de tubos múltiplos quantitativo ou número mais provável (NMP). Foram confirmados 100% de positividade para coliformes totais e coliformes termotolerantes em todas as amostras analisadas, com altas proporções registradas (≥ 1600 NMP/ml). Isto indica que essas águas são impróprias para o banho e consumo, representando possíveis transmissoras de doenças de veiculação hídrica. Dessa forma, é imprescindível uma avaliação regular da água do rio, assim como, buscar conscientizar o poder público e moradores locais sobre à preservação e à importância do rio para a Região do Cariri.
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