No abstract

Medicinal plants and its products have been used as a remedial agent in most irising countries for treating diseases. Furthermore, an increasing reliance on the use of medicinal plants in industrialized societies has been traced for the extraction and development of several drugs and chemotherapeutics from these herbal plants. Novel acetylenic fatty acids named Ximenynic acid (XMA) were successfully isolated from the seeds of Santalum album L by N-Hexane extraction. Ximenynic acid (or Santalbic acid) is one of the few acetylenic fatty acids occurring at higher levels in plant seed oils. Ximenynic acid predominantly exists in the seed oil of Santalaceae, Olacaceae, and Opiliaceae families. The structure of XMA was characterized by UV-visible spectroscopy, Infrared Spectrum(IR), NMR Spectroscopy, Differential scanning calorimetry (DSC), Thermal Gravimetric Analysis (TGA), X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy(FT-IR), LCMS spectral analysis. The antiaging activities were assessed by anti-collagenase enzyme assay. Structural analysis revealed that XMA was a crystalline material with a melting point of 38.25°C and an average molecular weight of 278 kDa.Which is composed of carboxylic acid, butylene acid, methylene, allylic in their structure. The antiaging assay showed that XMA exhibited significant collagenase inhibition activity as compared with Catechin. These findings suggested that the acetylenic fatty acids XMA could be served as a novel antiaging in Pharmaceutical as well as the cosmetic industry.

A new analytical approach and validation study were developed to assess the quantity of ximenynic acid (XMA) in Santalum album Linn. extract. High-performance liquid chromatography (HPLC) in reverse phase is the technique that is used in this procedure. The anti-aging properties of the medicine were tested using enzymes that inhibit hyaluronidase and elastase, respectively, in order to gather information and findings. Methanol and water were used as the mobile phase for the analysis, which was carried out using a ZORBAX SB-C18 column with a size 5 µ (4.6 x 250 mm) column size. During the course of this HPLC method validation, a number of different tests that were pertinent to specificity, linearity, accuracy, precision and robustness were carried out. The technique was tested to see whether or not it would be able to fulfill the prerequisites set out by the International Conference on Harmonization (ICH). Research conducted on anti-aging found that XMA was much more efficient than catechin at decreasing the activities of elastase and hyaluronidase. Because of its use, speed and reliability, the HPLC method that was described has the potential to be effectively utilized in industry for the standardization of herbs and phytomedicines. Because XMA has the potential to be a breakthrough anti-aging treatment, the pharmaceutical and cosmetics industries may benefit from its development.

Background These days, the presence of simple impurities in pharmaceuticals is a major cause for worry. This is because some contaminants are dangerous on their own, and even small impurities can make a drug less stable and shorten its shelf life. The goal of this study was to see if creams with ximenynic acid could be tested with inductively coupled plasma-mass spectrometry to find out how much arsenic, mercury, lead, cadmium, vanadium, cobalt, and nickel were in them. The best way to do things would be one that was quick, accurate, sensitive, and very productive (ICP-MS). The method included both inductively coupled plasma mass spectrometry (ICP-MS) and microwave digestion. Results Seven of the seven linearity correlation coefficient (‘R’) value were more than 0.99. LOD values were calculated using 33% of the 0.25 J threshold. Six LOQ responses (0.25 J level) were taken after considerable discussion. Calculated and reported %RSD for six LOQ copies. All elemental impurities Vanadium (V), Cobalt (Co), Nickel (Ni), Arsenic (As), Cadmium (Cd), Mercury (Hg), and Lead (Pb) were recovered between 83.33% and 115.97%, within acceptability limits. RSD% for procedure precision and intermediate precision data never exceeded 5%. The available evidence shows that the ICP-MS technique is a good way to measure these components. Conclusion The statistical analysis showed that the developed ICP-MS method for measuring elements in Topical Cream with Ximenynic Acid is selective and accurate. Since this ICPMS method is good at estimating several elements simultaneously, it could be used to check for elemental contaminants in the formulation.