Background: Perindopril erbumine (PE) and Indapamide (IND) in combination were proven to have a synergistic antihypertensive impact when compared with the use of each component alone. Objectives: Therefore, a new  simple,  selective, precise, and stability indicating RP- HPLC method for analysis of  Perindopril erbumine (PE) and Indapamide both in a bulk and in pharmaceutical formulation has been developed and validated. Methods: RP ‑ HPLC  method, Younglin (S.K.) Gradient System  UV Detector  and C18  column with 250mm  x 4.6 mm i. d. and 5μm particle size acetonitrile 70+  water with OPA 30 was used as the mobile phase for the method. The detection wavelength was 215 nm and flow rate was 1 ml/min. Results: In the developed method, the retention time of Perindopril erbumine (PE) and Indapamide were found to be 5.4833 min and 9.4500min. The developed method was validated according to the ICH guidelines. The linearity, precision, range, robustness was within the limits as specified by the ICH guidelines. Conclusion: Hence the method was found to be simple, accurate, precise, economic and reproducible. The forced degradation studies proved stability indicating power of the method.

A new analytical approach and validation study were developed to assess the quantity of ximenynic acid (XMA) in Santalum album Linn. extract. High-performance liquid chromatography (HPLC) in reverse phase is the technique that is used in this procedure. The anti-aging properties of the medicine were tested using enzymes that inhibit hyaluronidase and elastase, respectively, in order to gather information and findings. Methanol and water were used as the mobile phase for the analysis, which was carried out using a ZORBAX SB-C18 column with a size 5 µ (4.6 x 250 mm) column size. During the course of this HPLC method validation, a number of different tests that were pertinent to specificity, linearity, accuracy, precision and robustness were carried out. The technique was tested to see whether or not it would be able to fulfill the prerequisites set out by the International Conference on Harmonization (ICH). Research conducted on anti-aging found that XMA was much more efficient than catechin at decreasing the activities of elastase and hyaluronidase. Because of its use, speed and reliability, the HPLC method that was described has the potential to be effectively utilized in industry for the standardization of herbs and phytomedicines. Because XMA has the potential to be a breakthrough anti-aging treatment, the pharmaceutical and cosmetics industries may benefit from its development.

Objective: The day by day new combinations drugs are being introduced in market. Then the multiple therapeutic agents which acts at different sites are used in the management of various diseases and disorders are done. Thus it is necessary to develop methods for analysis with the help of number of analytical techniques which are available for the estimation of the drugs in combination. An  accurate,  precise  and  reproducible  RP-HPLC  method  was developed for the simultaneous quantitative determination of METO and BENI in tablet dosage forms. Methods: Younglin (S. K.) gradient system UV detector and C18 column with 250 mm x 4.6 mm i. d.  and 5μm particle size Acetonitrile: OPA water (45: 55v/v) pH 2.5 was used as the mobile phase for the method. The detection wavelength was 230 nm and flow rate was 1ml/min. Results: In the developed method, the retention time of Metoprolol and Benidipine were found to be 2.9833 min and 7.3833 min. The developed method was validated according to the ICH guidelines. Conclusion: The developed method was validated according to the ICH guidelines. In this methods linearity, precision, range, robustness was within the limits as specified by the ICH guidelines.

Background These days, the presence of simple impurities in pharmaceuticals is a major cause for worry. This is because some contaminants are dangerous on their own, and even small impurities can make a drug less stable and shorten its shelf life. The goal of this study was to see if creams with ximenynic acid could be tested with inductively coupled plasma-mass spectrometry to find out how much arsenic, mercury, lead, cadmium, vanadium, cobalt, and nickel were in them. The best way to do things would be one that was quick, accurate, sensitive, and very productive (ICP-MS). The method included both inductively coupled plasma mass spectrometry (ICP-MS) and microwave digestion. Results Seven of the seven linearity correlation coefficient (‘R’) value were more than 0.99. LOD values were calculated using 33% of the 0.25 J threshold. Six LOQ responses (0.25 J level) were taken after considerable discussion. Calculated and reported %RSD for six LOQ copies. All elemental impurities Vanadium (V), Cobalt (Co), Nickel (Ni), Arsenic (As), Cadmium (Cd), Mercury (Hg), and Lead (Pb) were recovered between 83.33% and 115.97%, within acceptability limits. RSD% for procedure precision and intermediate precision data never exceeded 5%. The available evidence shows that the ICP-MS technique is a good way to measure these components. Conclusion The statistical analysis showed that the developed ICP-MS method for measuring elements in Topical Cream with Ximenynic Acid is selective and accurate. Since this ICPMS method is good at estimating several elements simultaneously, it could be used to check for elemental contaminants in the formulation.

We developed and validated a simple, rapid, sensitive, precise, and reproducible specific UV spectrophotometric technique for determining Saquinavir in bulk medication and pharmaceutical dosage form. Methods: As per ICH recommendations, a simple double-beam UV spectrophotometric technique has been designed and validated with several criteria, such as linearity, precision, repeatability, the limit of detection (LOD), limit of quantification (LOQ), and accuracy. Results: UV-visible spectrophotometric approach, measurement of absorption at the maximum wavelength in 10 mL methanol, and volume made with water solvent system as reference Saquinavir was discovered at 239 nm. The medicine followed Beer's rule and showed a good correlation. Beer's law was followed for Saquinavir in the 1-5µg/ml concentration range, with a correlation coefficient of 0.999. Saquinavir's LOD and LOQ were determined to be 0.56 μg/ml and 2.78 μg/ml, respectively. The proposed method is simple, precise, accurate, and reproducible, and it may be utilized for routine analysis of Saquinavir in bulk and tablet dosage form.