Abstract:Terminalia arjuna is one of the many such plant that used in indigenous system of medicine for curing different diseases. For effective formulation of herbal drug selection of proper age of the plant is imperative. In the present study, the T. arjuna plant (leaves) was collected from the Botanical garden of Saurashtra University. The leaves were grouped in to various size and growth analysis was performed. Each extract homogenized in methanol and was used for chlorophyll estimation and antioxidant activities. Radical scavenging activity of the methanol extract of different stages leaves were determined using a stable free radical 2, 2-diphenyl-1-picryl hydrazyl (DPPH), using Micro plate assay. Further the phenolic compounds were separated by Reverse phase high pressure liquid chromatography analysis (RP-HPLC). At the leaf stage when maximum antioxidant activity was observed, phenol like Gallic acid showed distinct peak. The role of phenolic compounds in antioxidant activities is discussed.
Leaf area is an important parameter in physiology and agronomy studies. Linear models for leaf area measurement are developed for plant species as a nondestructive method. The plant Adhatoda vasica L. (a medicinal plant) was selected and the leaves of this plant were used for development of linear model for leaf area using Leaf Area Meter (LAM) software. Planimetric parameters (length, length2, width and width2) and gravimetric (dry weight and water content) parameters are considered for the development of linear model for this plant species. Single factor ANOVA and linear correlations were worked out using these parameters and leaf area. The plant was showed significant relationship with the parameters studied. The best correlation as represented by regression coefficient (R2) was used and improved R2 is worked out. It is observed that with increase in leaf area, water content is also increased and showed best correlation with the leaf area. Thus water content can be taken as a parameter for developing linear model for leaf area is concluded.
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