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Background and Objectives:Various bacterial species from subgingival biofilm have demonstrated aetiological relevance in the initiation and progression of periodontitis. The aim of this study was to detect the presence of Tannerella forsythia (Tf) in subgingival plaque of periodontally healthy subjects and chronic periodontitis patients by using both culture and PCR technique and compare the two techniques.Materials and Methods:Pooled subgingival plaque samples were taken using sterile curettes from predetermined sites in 50 periodontally healthy subjects and from 50 periodontitis subjects. Samples were analyzed for the presence of T. forsythia using both techniques. Statistical analysis of the results was done using Chi-square test, sensitivity, and specificity tests.Results:Both techniques could detect T. forsythia in subgingival plaque samples from healthy and periodontitis subjects. Periodontally healthy individuals and individuals with chronic periodontitis using the culture technique showed the presence of T. forsythia in 14 and 34%, respectively. PCR technique showed the presence of T. forsythia in 20% healthy and 40% chronic periodontitis patients. T. forsythia detection in the periodontitis group was statistically significantly higher when compared to the healthy group by both culture and PCR technique (P = 0.019 and P = 0.029). PCR demonstrated high sensitivity and low specificity when compared to the culture technique.Conclusion:The results indicated that T. forsythia was more prevalent in periodontitis patients when compared with healthy subjects. The PCR was found to be more sensitive than culture technique for detection of T. forsythia from the subgingival plaque samples.

Tumor-associated tissue eosinophil count is higher in WDSCC as compared to moderate and PDSCC.

Introduction: According to the World Health Organization (WHO) global burden of disease project, chronic kidney disease is the 12th leading cause of death and 17th leading cause of disability in the world. Statistics show that 90% of the patients suffering from chronic renal failure face oral health-related problems. The objective of the study was to assess the oral health status and treatment needs in hemodialysis patients at Raichur district, Karnataka. Materials and Methods: A cross-sectional study was carried out on 110 patients undergoing hemodialysis at Raichur district, Karnataka. Oral health status was assessed using the WHO assessment form 1997. Oral Hygiene Index – Simplified (1964) was used to assess the Oral Hygiene Status. Results: The mean age of the patients was 43.99 ± 12.80 years and the mean Decayed Teeth, Missing Teeth, Filled Teeth (FT), and Decayed Missing FT was 2.53 ± 2.39, 4.39 ± 7.57, 0.08 ± 0.30, and 7.00 ± 7.01, respectively. The prevalence of dental caries was 94.5%. Calculus was seen in 60.9% of the patients. Oral hygiene status was poor in 49.5% of the patients. The mean number of teeth required treatment was 4.4. Conclusion: This special population has high prevalence of dental caries and poor oral hygiene which needed periodic health education and intervention.

Introduction: Dental age (DA) assessment is a reliable method for chronological age (CA) estimation used for criminal, forensic, and anthropologic purposes. Age estimation also provides valuable information when the birth date is unavailable, as in case of migrants. Furthermore, the CA of living people is important for employment and marriage purpose. Objectives: The aim of the present study is to check the reliability of the Willems method in the population of Bangalore. Materials and Methods: The study was conducted on 205 (109 boys and 96 girls) participants by their radiographic records in the department of forensic odontology using modified Demirjian's method by Willem et al . Results: A total of 205 subjects were selected for the study, among which 109 (53.2%) were boys and 96 (46.8%) were girls. The mean CA was 12.23 ± 1.75 and the mean DA was 12.20 ± 1.99. Willems method seems to be reliable in estimating DA in Bangalore populations. Conclusion: This study showed that the Willems method gave near precise estimation of the CA.