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According to Biopharmaceutical classification system (BSC), candesartan cilexetil (CC) is a class-II drug which has limited bioavailability mainly due to its low solubility. In order to enhance its solubility, a universal approach of making nanosuspension is been used in the present investigation. High pressure homogenization, controlled precipitation, media milling and high speed homogenization are the various approaches which are most widely used to produce the nanosuspension. Here, nanosuspension is formulated by combination of two approaches; high speed homogenization and media milling to expedite and ease the process. The polymers used to stabilized the nanoparticles were polyvinylpyrrolidone K-30 (PVP K-30), poloxamer 407, HPMC E 50. To optimize the concentration of the polymer and surfactant the simplex lattice design is used. Various process parameters like homogenization speed, time, media milling cycle, drug to bead ration are optimized by changing one parameter at a time. The nanoparticles produced were of particle size less than 500 nm and were also found to be stable. The saturation solubility was enhaced more than 20 times than the bulk drug. The nanonization of the particles by combination of high speed homogenization and media milling is an effective method of enhancing in vitro dissolution of Candesartan cilexetil.

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Objective: This study is aimed to evaluate extraction capacity of different solvents (toluene, dichloromethane, chloroform, ethyl acetate, ethanol, methanol, water, 60% aqueous methanol, 60% aqueous ethanol and 60% aqueous acetone) and its effect on total phenolic content, total flavonoid content, and antioxidant assay. Methods: Extraction was performed from dried root powder of Boerhavia diffusa using various solvents at 25˚C on magnetic sirr (300 rpm). Extraction yield, total phenolic content, total flavonoid content and total tannin content of the extracts were determined spectrophotometrically using gallic acid, quercetin as standards. Antioxidant potential determines by using various in vitro methodologies such as DPPH, FRAP, and ABTS assay. Result: The 60% aqueous methanol showed the highest extracting yield, in contrast, toluene and hexane showed the lowest yield. Highest total phenolic content (239.8±0.25 mg GAE/g) and total flavonoid content (131.1±4.20 mg QCE/g), were found from methanolic extract. While, acetone extract showed highest tannin content. The Methanolic extract of Boerhaavia diffusa exhibited the highest antioxidant activity. Conclusions: The highest correlation was found between phenolic content and the antioxidant assay. It seems that phenolic contents are responsible for free radicle scavenging activity. From the observation, it concluded that methanolic extract rich with polyphenolic content and acetone extract showed the highest amount of tannin content.