The powder protein is mostly used as the main dietary by bodybuilders for improving exercise training. This research studied the effect of the powder protein intake on serum total protein and creatinine concentrations, and urine pH and protein of three different groups. The serum total protein concentration has no relationship with the amount of powder protein intake and cannot be affected by it. But the serum creatinine concentration has a direct relationship with the amount of powder protein intake. The group A and B serum creatinine concentrations in their blood were abnormal due to lack in the amount of protein intake in their daily life and exercise. The group C used sufficient amounts of powder protein 1.2-1.4 gm/Kg/day, which maintained their creatinine concentration. This quantity of protein intake for bodybuilders is not only safe, but also is improved the training adaptations in their excises training. The urine tests indicated no significant differences (P < 0.01) between their pH and at the same time the trace amount of protein in some of individuals urine was observed.

A particle of zinc sulfide (ZnS) was synthesized by the chemical coprecipitation method using zinc sulfate heptahydrate (ZnSO4), ammonium sulfate (NH4)2SO4 as a reactant, and thiourea as a stabilizer and capping agent. The optioned product characterized by electron dispersive X-ray spectroscopy that exhibits the presence of Zn and S elements. The average particle size of the ZnS nanoparticles determined using X-ray diffraction is about 4.9 nm. The ultraviolet–visible spectroscopy showed the blue shift in wavelength and the band gap was 4.33 eV, the surface morphology of the synthesized ZnS nanoparticles powder was studied by scan electron microscopy which was showed the irregular and some spherical shapes of ZnS in a nanosized range. The Fourier-transform infrared spectroscopy observed an absorption peck at 657.73 and 613.36 cm−1 that were assigned to the stretching mods of the Zn-S band. The different amounts of ZnS nanoparticle were applied as bactericidal against Staphylococcus aureus by disk diffusion method. It displayed activity against S. aureus bacteria, which was carried out in the absence of irradiation.

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