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Cow’s milk-intolerance is a digestive problem on people who not able to digest milk. This problem may relate to the variant of (3-casein (CSN2), especially A1, suggested due to (3-casomorphins (BCM-7) formation during enzymatic digestion, for that selecting cattle free BCM-7 become a concern to produce digestive friendly milk. This study aimed to differentiate A1 and A2 allele variant of CSN2 gene in selected population of Indonesian Holstein cattle. In total 70 cows DNA were collected, and fragment of CSN2 exon 7 which contain Single Nucleotide Polymorphism (SNP) rs43703011 and rs43703013 were amplified. Variant analysis was done by mutation site analysis using PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) with Mspl restriction enzyme and DNA sequence for confirmation. Result shows 2 allele variants of (3-casein that are B type, representing A1 family variant, and A2 in mutation site rs43703013C>G. We found A2 allele in the studied population is superior in frequency than A1 (0.916 vs. 0.084). Of that, 8.6% cattle were heterozygotes that is BA2 and 91.4% were homozygotes A2A2. Cattle which carry A1 allele variant should be excluded from dairy cattle breeding program for further milk production free of BCM-7.

Follicle Stimulating Hormone Receptor (FSHR) is a gene that has function to regulate reproductive performance by controlling oogenesis in females and also spermatogenesis in male. Since dairy cattle operation lies on the reproductive trait efficiency, therefore it’s became a logical reason to use FSHR genes as part of selection criteria. This study aimed to determine allele and genotype variation of FSHR gene in local Indonesian Friesian Holstein (IFH) dairy cattle in Boyolali District, Central Java. In total 20 IFH cattle we sampled for DNA source. Allele and genotype variation was determined by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using AluI restriction enzyme. The methods started with DNA extraction, PCR, and DNA digestion, followed by allele and genotype frequencies calculation. Two alleles were observed in this study, C and G with respective frequencies 0.75 and 0.25. According to that, we found three genotype combinations that are CC, CG, and GG, the frequencies were 0.56, 0.38, and 0.06, respectively. To use that result as a part of selection criteria in dairy cattle, especially in females, further association study with reproductive trait needs to be done.

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This study was aimed to analyze the genetic variation of bPRL (Bovine Prolactin) gene and its association to milk production in Indonesian Friesian Holstein (IFH) dairy cattle. In total 49 DNA from whole blood were extracted and followed with PCR-RFLP to distinguish the genotype difference between individual samples. The fragment of bPRL gene was amplified using pair of primer forward 5’-GAGTCCTTATGAGCTTGATTCTT-3’ and reverse 5’-GCCTTCCAGAAGTCGTTTGTTTTC-3’, resulting in 156 bp PCR product size. DNA digestion then performed using RsaI restriction enzyme that recognizes 5’-GT↓AC-3’ sites with blunt-end termini. According to the study, two genotypes were discovered that are AA and AB, with respective frequencies of 0.84 and 0.16. Based on the genotype grouping of milk production data, AB genotype yielded 4016.63±1344 kg while AA genotype yielded 3314.32±1153 Kg of milk per lactation. However, there is no statistical (P>0,05) difference between both genotypes on the amount of milk yielded. It is concluded that bPRL gene was not associated with milk production traits in the studied Indonesian Dairy Cattle population.