The microbicidal effect of UV light depends on the dose in both, disinfection processes and natural inactivation by the sunlight in surface water. Deviations of the time dose reciprocity are well known from chemical water disinfection whereas no data are available about this effect in UV inactivation in water. In a previous study we found that the UV inactivation behaviour of yeast strains does not follow the time dose reciprocity, insofar that longer exposure led to higher reduction of cultivable cells. In contrast, an earlier study about E coli B/r claimed a higher inactivation with single exposure compared with fractionated UV irradiation. To investigate this question we selected water-relevant microorganisms and studied their UV inactivation behaviour (253.7nm) by means of a specially designed UV irradiation apparatus (a) under standard irradiation conditions (2W/m2) and (b) with three levels of UV dose rate (2, 0.2 and 0.02W/m2). The test organisms were (i) three E coli strains (ATCC 25922, ATCC 11229 and an isolate from sewage) representing the routinely used faecal indicator, (ii) three bacterial viruses (MS2, ϕX174 and B40-8) proposed as indicators for viral contamination in water and (iii) spores of Bacillus subtilis because of their use as a biodosimeter in prototype testing of commercial UV plants for drinking water disinfection. We found, under standard inactivation conditions, that the E coli strains and phage ϕX174 are most UV susceptible, followed by B40-8 and finally MS2 and bacterial spores. The dose protraction experiments revealed for the E coli strains a higher inactivation with high dose rates compared to low dose rates at the same UV doses (difference of about 1 log10 at 80-100J/m2). The other test organisms did not deviate from the time dose reciprocity in the proven range of dose.

Over the past 15 years, pioneering interdisciplinary research has been performed on the microbiology of hydrogeologically well‐defined alpine karst springs located in the Northern Calcareous Alps (NCA) of Austria. This article gives an overview on these activities and links them to other relevant research. Results from the NCA springs and comparable sites revealed that spring water harbors abundant natural microbial communities even in aquifers with high water residence times and the absence of immediate surface influence. Apparently, hydrogeology has a strong impact on the concentration and size of the observed microbes, and total cell counts (TCC) were suggested as a useful means for spring type classification. Measurement of microbial activities at the NCA springs revealed extremely low microbial growth rates in the base flow component of the studied spring waters and indicated the importance of biofilm‐associated microbial activities in sediments and on rock surfaces. Based on genetic analysis, the autochthonous microbial endokarst community (AMEC) versus transient microbial endokarst community (TMEC) concept was proposed for the NCA springs, and further details within this overview article are given to prompt its future evaluation. In this regard, it is well known that during high‐discharge situations, surface‐associated microbes and nutrients such as from soil habitats or human settlements—potentially containing fecal‐associated pathogens as the most critical water‐quality hazard—may be rapidly flushed into vulnerable karst aquifers. In this context, a framework for the comprehensive analysis of microbial pollution has been proposed for the NCA springs to support the sustainable management of drinking water safety in accordance with recent World Health Organization guidelines. Near‐real‐time online water quality monitoring, microbial source tracking (MST) and MST‐guided quantitative microbial‐risk assessment (QMRA) are examples of the proposed analytical tools. In this context, this overview article also provides a short introduction to recently emerging methodologies in microbiological diagnostics to support reading for the practitioner. Finally, the article highlights future research and development needs.This article is categorized under: Engineering Water > Water, Health, and SanitationScience of Water > Water ExtremesWater and Life > Nature of Freshwater Ecosystems

The effective UV-dose in a UV-reactor depends on the transmission and the flow rate of the water being irradiated as well as on the design of the plant and the hydraulic behavior. Since there is no possibility for a direct and simple dose measurement in such a system, it is necessary to develop a testing procedure which estimates the hygienic safety of such a plant. B. subtilis (ATCC 6633) spores were suspended in potable water and irradiated in a commercially available drinking water UV plant with varying flows (0.5 to 7.5 m3/h) and transmission (3 to 80 %; thickness 10 cm). Sodium thiosulfate and adenosine were used as transmission reducing substances. Because of the known UV sensitivity of the spores it was possible to calculate the effective dose in the reactor as space radiant exposure for each test. The data resulted in an individual diagram of efficiency. It was shown that this testing procedure is suitable for the evaluation of the disinfection capacity of UV plants and the estimation of the field of application depending upon transmission and flow rate of the water being irradiated.

DNA aptamers generated by cell-SELEX against bacterial cells have gained increased interest as novel and cost-effective affinity reagents for cell labelling, imaging and biosensing. Here we describe the selection and identification of DNA aptamers for bacterial cells using a combined approach based on cell-SELEX, state-of-the-art applications of quantitative real-time PCR (qPCR), next-generation sequencing (NGS) and bioinformatic data analysis. This approach is demonstrated on Enterococcus faecalis (E. faecalis), which served as target in eleven rounds of cell-SELEX with multiple subtractive counter-selections against non-target species. During the selection, we applied qPCR-based analyses to evaluate the ssDNA pool size and remelting curve analysis of qPCR amplicons to monitor changes in pool diversity and sequence enrichment. Based on NGS-derived data, we identified 16 aptamer candidates. Among these, aptamer EF508 exhibited high binding affinity to E. faecalis cells (KD-value: 37 nM) and successfully discriminated E. faecalis from 20 different Enterococcus and non-Enterococcus spp. Our results demonstrate that this combined approach enabled the rapid and efficient identification of an aptamer with both high affinity and high specificity. Furthermore, the applied monitoring and assessment techniques provide insight into the selection process and can be highly useful to study and improve experimental cell-SELEX designs to increase selection efficiency.