AIM: To evaluate the immunomodulatory effects of ethanolic extract of herb pugun tanoh on TCD4 and TCD8 cells in Doxorubicin-induced rats. METHODS: Fifteen male Sprague Dawley rats were divided into five groups consisting of six rats each as follows: Group 1, DOX-treated rats (4.67 mg/kg BW body weight on day 1 and 4) and were administered normal saline 0.9% orally once daily for 7 consecutive days, Group 2, receiving Ethanolic Extract of Herb Pugun Tanoh (Picria fel-terrae Lour.) of dose 150 mg/kg BW orally, Group 3, receiving dose Ethanolic Extract of Herb Pugun Tanoh (Picria fel-terrae Lour.) 300 mg/kg BW orally. The rats of group 2-3 were intramuscularly administered with doxorubicin at a dose of 4.67 mg/kg BW at the days 1-4 to suppress immune functions. RESULTS: Treatment of 300 mg/kg BW of Ethanolic Extract of Herb Pugun Tanoh (Picria fel-terrae Lour.) succeeded in reducing side effect doxorubicin based on increasing the TCD4+ and TCD8+ blood level. CONCLUSION: Ethanolic Extract of Herb Pugun Tanoh (Picria fel-terrae Lour.) could increase the level of TCD4+ and TCD8+ in rats which induced by doxorubicin.

Objective: The objective of this study is to assess the cytoprotective activity of Coleus amboinicus Lour. leaves ethanol fraction (EtF) in Vero cells which induced by hydrogen peroxide (H2O2).Methods: Cytoprotective activity was determined using 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis activity was analyzed by flow cytometry, and expression of radical spesies oxygen (ROS) was observed by imunocytochemistry.Result: The EtF (100 μg/mL) was showed the highest percent viability 74.42±0.28% using MTT method. Furthermore, the EtF was increased viability of Vero cell after induced with 0.8 mM H2O2 and EtF was decreased the expression of ROS.Conclusion: Based on the description, EtF has cytoprotective activity towards Vero cells which induced by 0.8 mM H2O2 .

Objective: The purpose of this study was to determine the physical stability and effectiveness of hair growth in hair tonic preparations containing ethanol extract of clove leaves (Syzygium aromaticum) with a concentration variation of 5, 10, and 15%. Methods: Formulations were evaluated, including organoleptic observation, pH checking, and preparation stability tests using the Climatic Chamber. The effectiveness of clove leaf extract as a hair tonic was tested by looking at hair growth in test animals. Results: The results showed that the preparation was stable at high temperatures (40±2 °C) where the odor, color, clarity, and pH remained constant. Effectiveness test on rabbit hair growth showed significantly different results after measuring hair growth on the 21st day, where the results were 11.83+0.06 mm for positive control, 1.71+0.06 mm as blank, 1.69+0.06 mm for the group without treatment, and successively the results of the group given clove leaf extract 5, 10, and 15% were 4.00+0.06; 4.80+0.06; and 5.46+0.08 mm. Conclusion: Hair tonics containing clove ethanol extract can increase rabbit hair growth.