Background: Avocado is a plant that can grow in tropical climates. In general, people only consume the fruit while the skin and seeds are not used. Avocado fruit, skin and seeds contain secondary metabolites such as flavonoids and tannins which have antioxidant activity. Objective: To determine antioxidant activity, total flavonoid, and total tannin content of the ethanol extract of avocado peel. Methods: P. americana Mill. was collected from Lubuk Linggau, South Sumatra. The ethanol extract of P. americana Mill. peel obtained by maceration with ethanol. Qualitative analysis of flavonoids using Mg and HCl while tannins using FeCl3. Determination of total flavonoid content was carried out with AlCl3 reagent and Folin-Ciocalteu reagent to determine the total tannin content and was measured by a UV-Vis Spectrophotometer. Determination of antioxidant activity using the DPPH method. Result: The results of the qualitative analysis showed that ethanol extract of avocado peel contained flavonoids and tannins. It also had antioxidant activity with IC50 of 41,93 µg/mL. The total flavonoid and tannin content of it were 125,01 mgQE/g extract and 53,82 mgTAE/g extract respectively. Conclusion: Ethanol extract of avocado peel had antioxidant activity, while the total flavonoid compounds was higher than the total tannins. Keywords: antioxidant, DPPH, total flavonoid, total tannins
Nangka merupakan salah satu tanaman yang digunakan sebagai obat herbal, salah satunya untuk penyakit infeksi. Tujuan penelitian untuk mengetahui aktivitas antibakteri ekstrak n-heksan, kloroform dan etanol daun nangka (Artocarpus heterophyllus Lam.) Ekstraksi dilakukan dengan cara beringkat dengan metode sokhletasi. Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi agar. Bakteri uji yang digunakan adalah Propionibacterium acnes ATCC 11827 dengan konsentrasi ekstrak 10.000 µg/mL, 5.000 µg/mL. 2.500 µg/mL dan 1.250 µg/mL. Uji aktivitas antibakteri ekstrak n-heksan, kloroform dan etanol daun nangka menunjukkan ada diameter hambat. Pada ekstrak n-heksan, kloroform dan etanol daun nangka diperoleh diameter hambat paling besar berturut-turut 12,26 mm, 12,36 dan 12,26 mm. Uji fitokimia dengan KLT menunjukkan bahwa ekstrak n-heksan mengandung terpenoid dan steroid, ekstrak kloroform mengandung fenol, dan ekstrak etanol mengandung flavonoid. Hal ini menunjukkan bahwa senyawa aktif pada ekstrak mampu menghambat pertumbuhan Propionobacterium acnes ATCC 11827.
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