The antioxidant and cytotoxic activities of the chloroform extract of agarwood (Gyrinops versteegii (Gilg.) Domke) leaves on HeLa cell lines

Nuringtyas, Tri Rini; Isromarina, Rini; Septia, Yovi; Hidayati, Lisna; Wijayanti, Nastiti; Moeljopawiro, Sukarti

doi:10.1063/1.5050163

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…CITES (The Convention on International Trade in Endangered Species of Wild Flora dan Fauna) juga mengelompokan status konservasi G. versteegii ke dalam daftar appendix II. Padahal penelitian mengenai G. versteegii semakin banyak dilakukan dan didukung oleh kemampuan bioaktivitasnya sebagai antioksidan (Nuringtyas et al, 2018), antikanker (Nuringtyas et al, 2018;Wardana et al, 2019) dan antivirus (Masita et al, 2020).…”

Section: Pendahuluanunclassified

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa

Hidayati

Sasongko

et al. 2021

JBT

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Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.

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Section: Pendahuluanunclassified

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa

Hidayati

Sasongko

et al. 2021

JBT

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“…Perkebunan G. versteegii dapat ditemukan di wilayah Sumatera, Jawa dan Kalimantan dengan kemelimpahan yang lebih rendah dibandingkan persebarannya di wilayah Timur sebagai habitat alaminya (Rindyastuti et al, 2019). Penelitian ilmiah tentang potensi Gyrinops versteegii sebagai herbal telah dilaporkan oleh Nuringtyas et al, (2018) yang menunjukkan daun G. versteegii memiliki aktivitas antioksidan dan anti proliferasi sel kanker serviks (HeLa) yang tinggi. Profil metabolit daun G. versteegii dianalisa dengan GC-MS menunjukkan bahwa ekstrak kloroformnya memiliki 15 metabolit yang berpotensi sebagai anti kanker (Wardana et al, 2019).…”

Section: Pendahuluanunclassified

Metabolite profiling of agarwood (Gyrinops versteegii (Gilg.) Domke) leaves from difference growth locations using Thin Layer Chromatography

Manurung

Hidayati

Wijayanti

et al. 2021

JBT

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Gyrinops versteegii (Gilg) Domke is an agarwood-producing plant originating from Eastern Indonesia. Leaves have antioxidant and anticancer activity due to their secondary metabolites. However, secondary metabolites are strongly influenced by the environment. This study aims to compare the terpenoid, phenolic, and flavonoid profiles of agarwood leaves grown in three locations, namely Bogor, Mataram, and Sambas using Thin Layer Chromatography (TLC), in order to obtain the most potential agarwood G. versteegii for further development as herbal medicine. Agarwood leaf powder was extracted using Soxhlet with 80% methanol as solvent, followed by liquid-liquid fractionation using chloroform and aquadest. The crude extract and the obtained fraction were subjected to metabolite profiling using TLC. Racing data factors from TLC were analyzed using hierarchical cluster analysis (HCA). The results showed that the Mataram sample produced higher yields for both extracts and fractions. TLC detected that the chloroform fraction contained more terpenoids, followed by phenolics and flavonoids, while the aqueous fraction contained the same number of positive bands for terpenoids and phenolics. In terms of origin, the Bogor sample showed six terpenoid positive bands higher than the other samples. A similar pattern was observed in the phenolics, while the flavonoids were the same for all samples. HCA resulted in Mataram being clustered separately from the Bogor and Sambas samples. Agarwood leaves from Mataram cannot be used to replace agarwood leaves from Bogor and Sambas as herbal ingredients.

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“…Data studi fitokimia tersebut dapat dijadikan sebagai dasar yang kuat untuk mengembangkan produk berbasis daun gaharu di level industri terutama yang melibatkan kombinasi dengan berbagai bahan herbal lainnya Skrining awal terhadap ekstrak daun Gyrinops menunjukkan bahwa senyawa yang terkandung didalamnya tidak jauh berbeda dengan senyawa yang terdapat dalam daun Aquilaria. Berdasarkan hasil Fraksinasi senyawa dengan metode kromatografi lapis tipis, ekstrak ini mengandung komponen kelompok senyawa Phenolic, Flavonoid, Tannin dan Terpenoid (Nuringtyas et al, 2018)…”

Section: Fitokimia Daun Gaharuunclassified

“…Fraksinasi lebih lanjut terhadap senyawa yang memiliki aktivitas cytotoxic dengan menggunakan metode kromatografi lapis tipis menunjukkan bahwa fraksi tersebut mengandung senyawa Fenol, Flavonoid dan Terpen. (Nuringtyas et al, 2018) Aktivitas cytotoxic dari daun G. versteegii berkorelasi dengan kemampuannya sebagai bahan baku senyawa antikanker. Ekstrak kloroform daun G. versteegii terbukti memiliki kemampuan mencegah metastasis terhadap cell line kanker payudara T47D.…”

Section: Khasiat Medis Daun Gyrinopsunclassified

Medicinal Effect Review of Agarwood Leaves From Aquilaria and Gyrinops Genera

Wangiyana

2020

JSS

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Aquilaria and Gyrinops are the most common genera of Agarwood in Indonesia. These two group have wide distribution in Indonesia. Therefore, Indonesia is one of the biggest agarwood commodity producer in the world. However, the resin on agarwood stem is still main utilization of agarwood commodity in this country. Diversification agarwood utilization other than resin is needed to maximilize the potency of this commodity. Agarwood leaves are one of by product from agarwood cultivation that rarely been utilized. Agarwood leaves contain secondary metabolites that have medical effect such as Phenol, Flavonoid, Terpenoid , Alkaloid and Tannin. Thus, agarwood leaves has a good potency as a source of food and beverage product that have a medicinal effects. Agarwood leaves form Aquilaria group and Gyrinops group both have medicinal effect including antioxidant, antibacterial, cytotoxic and anticancer. Aquilaria leaves also has additional medicinal effect including: anti-inflammatory, anti-diabetic and laxative while Gyrinops leaves also has additional medicinal effect as antiradical source of sunscreen.

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The antioxidant and cytotoxic activities of the chloroform extract of agarwood (Gyrinops versteegii (Gilg.) Domke) leaves on HeLa cell lines

Cited by 9 publications

References 17 publications

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Metabolite profiling of agarwood (Gyrinops versteegii (Gilg.) Domke) leaves from difference growth locations using Thin Layer Chromatography

Medicinal Effect Review of Agarwood Leaves From Aquilaria and Gyrinops Genera

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