BackgroundMycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.ResultsOf the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.ConclusionThe microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products.

Rhodococcus equi is a well-recognized Gram-positive intracellular facultative bacterium that is opportunistic in nature, which causes pyogranulomatous infections in humans and multiple host animals. The pathogenicity of the microorganism has been attributed to the presence of plasmid-encoded virulence-associated proteins (Vap). To date, three host-associated virulence plasmid types of R. equi have been identified as follows: the circular pVAPA and pVAPB, related, respectively, to equine and porcine isolates, and a recently described linear pVAPN plasmid associated with bovine strains, although these three types are found in human isolates. Recent phylogenomic studies support the evidence that human R. equi infection is zoonotically acquired. Nevertheless, data regarding distribution and prevalence of the host-adapted virulence plasmid types of R. equi isolated from meat animals are scarce or unnoticed. Here, the three host-associated virulence plasmid types (pVAPA, pVAPB, and pVAPN) were investigated in 154 R. equi isolates recovered from lymph nodes of cattle with lymphadenitis (n = 31), faeces of cattle without enteric signs (n = 49), as well as different clinical specimens from human patients (n = 74). The analysis of virulence profile of 74 R. equi from humans revealed six (8.1%) isolates pVAPB (type 8), two (2.7%) pVAPN, and one (1.3%) pVAPB (type 11), all of which were from lung samples from people living with HIV/AIDS. From the lymph node samples of cattle, 41.9% (13 of 31) isolates revealed pVAPN type, whereas all isolates from faecal samples were negative for three host-associated types. Here, recently described bovine-associated pVAPN type was detected in R. equi isolates recovered from the lungs of people living with HIV/AIDS and lymph nodes from slaughtered cattle intended for human consumption; a finding that represents a public health concern, mainly in countries where undercooked or raw meat are traditionally consumed.

Resumo:O leite informal é reconhecido como o produto comercializado sem inspeção. Avaliou-se a qualidade microbiológica, constituintes nutricionais e presença de substâncias inibidoras do crescimento bacteriano em 100 amostras informais de leite de vacas, comercializadas na região sudoeste do estado de São Paulo, Brasil. Verificou-se que 77% da Contagem de Células Somáticas (CCS) e 86% da Contagem Bacteriana Total (CBT) das amostras estavam em desacordo com os valores máximos exigidos pela Instrução Normativa 62 do MAPA. Identificaram-se teores em desacordo para sólidos totais (36%), proteína (23%), gordura (38%), extrato seco desengordurado (43%) e nitrogênio uréico (73%). Em 59% das amostras detectou-se a presença de resíduos de inibidores do crescimento bacteriano. Isolaram-se 240 linhagens de micro-organismos com predomínio de enterobactérias, estreptococos, estafilococos e fungos. O leite informal é comercializado na região sem as mínimas condições de higiene e oferece riscos a saúde pública, além de representar sério problema sócio-econômico para região.

Coxiella burnetii is a zoonotic pathogen with a worldwide distribution that is responsible for Q fever in humans. It is a highly infectious bacterium that can be transmitted from cattle to humans through the consumption of unpasteurized milk. We report the molecular identification of C. burnetii in raw cow's milk being sold directly for human consumption in Brazil without official inspection or pasteurization. One hundred and twelve samples of raw milk were analysed by real‐time quantitative PCR (qPCR), and C. burnetii was detected in 3.57% (4/112) of the samples at a concentration ranging from 125 to 404 bacteria per millilitre. The identification of this zoonotic pathogen in raw milk sold directly for human consumption is a public health concern since C. burnetii can be transmitted through the oral route. This result indicates that health education and other preventive measures should be officially implemented in Brazil to prevent the spread of infection. To our knowledge, this is the first qPCR‐based detection of C. burnetii in raw milk samples from cows sold in Brazil that do not undergo official inspection or pasteurization.

This article analyzes opinion polls conducted by the Brazilian Institute of Public Opinion and Statistics (IBOPE) in the context of 1964, with the objective of assessing the support to the coup and to dictatorship. The data, mostly new, indicate a contrast between the support to João Goulart, registered before the coup, and after the success of the coup, which points out to the good acceptance of authoritarian measures, including political purges. The empirical data obtained from the polls are used to consider the sources of legitimation of dictatorship that especially mobilized anticommunist representations. The analysis of the records suggests that the support to the authoritarian regime was marked by instability and oscillated throughout the initial years. Keywords: dictatorship; IBOPE; 1964 coup. O golpe de 1964 e a ditadura nas pesquisas de opinião ResumoO artigo analisa pesquisas de opinião realizadas pelo Instituto Brasileiro de Opinião Pública e Estatística (IBOPE) no contexto de 1964, com o objetivo de avaliar o apoio ao golpe e à ditadura. Os dados, na maioria inéditos, apontam um contraste entre o apoio a João Goulart, registrado antes do golpe, e os números colhidos depois da vitória golpista, que indicam boa aceitação das medidas autoritárias, inclusive os expurgos políticos. O material empírico resultante das enquetes é utilizado para refletir sobre as fontes de legitimação da ditadura que mobilizaram, sobretudo, representações anticomunistas. A análise dos registros sugere que o apoio ao regime autoritário foi marcado pela instabilidade e oscilou no decorrer dos primeiros anos. Palavras-chave: ditadura; IBOPE; golpe de 1964. El golpe de 1964 y la dictadura en los sondeos de opinión ResumenEl artículo analiza los sondeos de opinión realizados por el Instituto Brasileño de Opinión Pública y Estadística (IBOPE) en el contexto de 1964, con el objetivo de evaluar el apoyo al golpe y a la dictadura. Los datos, en su mayoría inéditos, señalan un contraste entre el apoyo a João Goulart, apuntado antes del golpe, y los números recogidos después de la victoria golpista, que indican la buena aceptación de medidas autoritarias, incluyendo purgas políticas. El material empírico resultante de los sondeos se utiliza para reflexionar sobre las fuentes de legitimidad de la dictadura, que movilizaron principalmente representantes anticomunistas. El análisis de los registros insinúa que el apoyo al régimen autoritario se caracterizó por la inestabilidad y fluctuó durante los primeros años. Palabras clave: dictadura; IBOPE; el golpe de 1964. Le matériel empirique originaire des enquêtes est utilisé pour réfléchir sur les sources de légitimation de la dictature qui ont notamment mobilisé les représentations anticommunistes. L' analyse des registres suggère que le soutien au régime autoritaire a été marqué par l'instabilité et a oscillé au cours des premières années. Mots-clés: dictature; IBOPE; coup d'État de 1964. Le coup d'État de 1964 et la dictature dans les sondages d'opinion