ABSTRAKFungi endofit dimanfaatkan sebagai sumber daya baru untuk menghasilkan senyawa bioaktif seperti antibakteri, antioksidan dan antikanker. Penelitian ini bertujuan menganalisis daya antibakteri, antioksidan, sitotoksik fraksi hasil pemisahan ekstrak etil asetat dari miselium fungi endofit genus Fusarium sp. Fraksi dietil eter menunjukan aktivitas antibakteri yang tinggi dengan nilai IC 50 pada bakteri E.coli (20,75 µg/mL), S.typhi (35,08 μg/mL), dan S.aureus (51,96 µg/mL). Uji aktivitas antioksidan menunjukan bahwa fraksi etanol 96% mempunyai aktivitas antioksidan dan kandungan fenolik total yang paling tinggi (75,85 ± 0,11 mg GAE). Uji sitotoksik pada sel kanker payudara jenis T47D menunjukan fraksi dietil eter mempunyai aktivitas tertinggi dengan IC 50 sebesar 10,16±0,88 µg/mL dibandingkan fraksi n-heksan dan etanol.Kata kunci: antibakteri, antioksidan, fungi endofit, kandungan fenolat total, sitotoksisitas ABSTRACT Endophytic fungi used as a new resource to produce bioactive compounds such as antibacterial, antioxidant and anticancer. This study aimed to analyze the antibacterial, antioxidant, cytotoxic fraction from separation ethyl acetate extract of mycelium of endophytic fungi genus Fusarium sp. Diethyl ether fraction showed high antibacterial activity in E.coli (20.75 µg/mL), S.typhi (35.08 µg/mL), and S.aureus (51.96 µg/mL). Test showed that the antioxidant activity of ethanol 96% fraction has highest antioxidant activity and total phenolic content (75.85 ± 0.87 mg GAE). Cytotoxic test on T47D breast cancer cells showed that the fraction of diethyl ether have highest activity with IC 50 of 10.16 ± 0.88 µg /mL compared to n-hexane and ethanol fraction.

Endophytic fungi can produce compound that similar with the host, so it can be used as mass production of compound. The optimal and valid assay method is needed to obtain the proper culture condition, which one using densitometric Thin Layer Chromatography (TLC). This research used 11 samples of endophytic fungi culture extract from Phyllantus niruri within various culture condition. Optimization of mobile phase was done using 3 kinds of mobile phase mixture, chloroform: ethyl acetate: methanol (8:8:4); chloroform: ethyl acetate: methanol: NH 4 OH (8:8:4:0.005); chloroform:ethyl acetate:NH 4 OH (8:8:0.005). Validation method measured using some parameters such as linearity, accuracy, and precision. The result of optimization and validation showed that TLC densitometry method can be used for measuring alkaloid level within the endophytic fungi extract of P. niruri using chloroform: ethyl acetate: NH 4 OH (8:8:0.005), with R value is 0.977, percentage of recovery is between 90-110, and RSD ≤7% on each concentration series. While the proper condition of endophytic fungi culture is using sucrose as carbon source and at pH 6 condition. The content of alkaoid reached 25.9 ± 1.4 mg 100 g -1 fresh weight.