Background: The evolution of nosocomial infections by multidrug resisitance (MDR) and extensive drug resistance (XDR) Acinetobacter baumannii and klebsiella pneumoniae are considered a major health problem owing to the relatively limited treatment options. Colistin and tigecycline are increasingly used as a last choice for treatment of these infections. The most accurate antibiotic susceptibility methods for colistin and tigecycline are still challenging. Objectives: The aim of the current study was to detect colistin and tigecycline antibiotic susceptibility of K. pneumoniae and A. baumanii and evaluate disk diffusion (DD), E- test and VITEK 2 automated system compared to broth dilution (BD) test. Methodology: This study was performed on 35 K. pneumoniae and 15 A. baumanii clinical isolates collected from patients admitted to Benha University Hospitals. The isolated strains were identified by the standard laboratory technique with subspecies identification by VITEK 2 automated system. Colistin and tigecycline antibiotic susceptibility for K. pneumoniae and A. baumanii were evaluated by E-test, disk diffusion and VITEK 2 compared to BD as the reference method. Results: Through the study of the studied k. pneumoniae and A. baumanii strains, The essential and categorical agreements of colistin suscebtibility were (82% & 80 %) for E-test, (92% & 98%) for VITEK 2 and categorical agreement for DD was 54%. The essential and categorical agreements of tigecycline suscebtibility were (96% & 98%) for E-test, (88% &78%) for VITEK 2 and categorical agreement for DD was 74%. Conclusion: For colistin, VITEK 2 is considered a reliable method to detect colistin susceptibility while E-test and disk diffusion showed a poor performance. For tigecycline, E-test showed the best performance compared to the gold standard test while shortcomings of automated VITEK 2 and manual DD were observed.

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Campylobacter jejuni (C.jejuni) , Campylobacter coli (C.coli), modified charcoal cefoperazol dextrose agar(MccDA) Background:Campylobacter is an important food-borne diarrheal disease in the whole world. The use of cultureindependent diagnostic tests , such as stool antigen tests, for the detection of Campylobacter in stool is of particular interest.Objectives:This study aimed to compare between conventional culture method and Immunochromatography (I.C) For Detection Of C.jejuni and C. coli in Gastroenteritis at Benha University hospitals, and determine their antibiotic susceptibility pattern.Methodology: This study was carried out on 100 patients who developed gastroenteritis like symptoms attending the out patient clinic of Benha University Hospitals or admitted to the Pediatric and Internal medicine departments. Stool samples were cultured on modified charcoal cefoperazol dextrose agar (mCCDA) and were examined for Ag detection by I.C test using RIDA®QUICK kit .Antimicrobial susceptibility testing was performed for twelve different antibiotics by the Kirby-Bauer disc diffusion method. Results: Campylobacter was detected in 18% of samples by culture method. 72.2% of them were C. jejuni, while the other 27.8% were C. coli. Out of 18 positive by culture ,16(88.9%) were positive by I.C. However, out of 82 negative by culture , 2(2.4%) were positive by I.C. The diagnostic accuracy values of the RidaQuick Campylobacter® versus culture were: sensitivity of 88.9%, specificity of 97.6%, and positive and negativepredictive values of 88.9% and 97.6%, respectively. Conclusion: RIDAQUICK Campylobacter®provide a rapid and reliable alternative for conventional culture in laboratory diagnosis of enteric infections with C. jejuni and C. coli.