Background: Hypertension is an important public health issue in developed and developing countries. The incidence of hypertension continues to rise to a serious level. Raising awareness of the seriousness of hypertension among peer groups may be an important factor for preventive health behavior. This study aimed to examine the used of health belief model for the analysis of factors affecting hypertension preventive behavior among adolescents. Subjects and Method: This study was an observational analytic study with cross sectional design. It was conducted at 5 Vocational High Schools (SMK) in Surakarta from April to May, 2017. A sample of 200 class X and XI SMK students aged 15-17 years was selected for this study by stratified random sampling. The dependent variable was hypertension preventive behavior. The independent variables were perceived susceptibility, perceived seriousness, perceived benefit, perceived barriers, cues to action, and self efficacy, with perceived threat as a mediating variable. The data were collected by a set of pre-tested questionnaire. Path analysis was employed for data analysis using SPSS AMOS 22. Results: Perceived threat (b=0.24, SE=0.07, p=0.002), perceived benefit (b=0.24, SE=0.10, p=0.021), self efficacy (b=0.40, SE=0.23, p=0.084), and cues to action (b=0.45, SE=0.15, p=0.003) showed direct positive effects on hypertension preventive behavior. Perceived barrier (b=-0.26, SE=0.10, p=0.015) showed direct negative effect on hypertension preventive behavior. Perceived susceptibility (b= 0.27, SE= 0.09, p=0.005), perceived seriousness (b=0.29, SE=0.09, p<0.001), and cues to action (b=0.34, SE=0.13, p=0.008) showed indirect positive effects on hypertension preventive behavior. Conclusion: Hypertension preventive behavior is positively and directly affected by perceived threat, perceived benefit, self, and cues to action. The preventive behavior is negatively and directly affected by perceived barrier. Perceived susceptibility, perceived seriousness, and cues to action indirectly and positively affect on hypertension preventive behavior.

Tenaga kesehatan terutama mahasiswa Ahli Teknologi Laboratorium Medik (ATLM) memiliki risiko lebih besar tertular infeksi virus hepatitis B dari paparan cairan tubuh ketika melakukan praktikum klinik. Pentingnya seroprevalensi, pengetahuan dan sikap sangat mempengaruhi mahasiswa saat melakukan praktikum sebagai tindakan pencegahan infeksi hepatitis B. Penelitian ini berujuan untuk mengidentifikasi seroprevalensi, pengetahuan dan sikap preventif mahasiswa ATLM terhadap infeksi virus hepatitis B. Penelitian ini merupakan penelitian deskriptif kuantitatif menggunakan desain studi cross sectional yang dilaksanakan disalah satu Perguruan Tinggi kesehatan di Surakarta pada bulan Oktober – Nopember 2018. Pengambilan subjek menggunakan teknik total sampling dengan jumlah responden sebanyak 71 mahasiswa program studi D-IV Teknik Laboratorium Medik. Pengumpulan data dilakukan dengan kuesioner tentang pengetahuan, dan sikap preventif infeksi hepatitis B pada mahasiswa serta sampel darah dikumpulkan dan diskrining dengan pemeriksaan HBsAg. Analisis data menggunakan komputerisasi dengan SPSS versi 21.

Background: Hypertension is an important public health issue in developed and developing countries. The incidence of hypertension continues to rise to a serious level. Raising awareness of the seriousness of hypertension among peer groups may be an important factor for preventive health behavior. This study aimed to examine the used of health belief model for the analysis of factors affecting hypertension preventive behavior among adolescents. Subjects and Method: This study was an observational analytic study with cross sectional design. It was conducted at 5 Vocational High Schools (SMK) in Surakarta from April to May, 2017. A sample of 200 class X and XI SMK students aged 15-17 years was selected for this study by stratified random sampling. The dependent variable was hypertension preventive behavior. The independent variables were perceived susceptibility, perceived seriousness, perceived benefit, perceived barriers, cues to action, and self efficacy, with perceived threat as a mediating variable. The data were collected by a set of pre-tested questionnaire. Path analysis was employed for data analysis using SPSS AMOS 22. Results: Perceived threat (b= 0.24, SE= 0.07, p= 0.002), perceived benefit (b= 0.24, SE= 0.10, p= 0.021), self efficacy (b= 0.40, SE= 0.23, p= 0.084), and cues to action (b= 0.45, SE= 0.15, p= 0.003) showed direct positive effects on hypertension preventive behavior. Perceived barrier (b= -0.26, SE= 0.10, p= 0.015) showed direct negative effect on hypertension preventive behavior. Perceived susceptibility (b= 0.27, SE= 0.09, p= 0.005), perceived seriousness (b= 0.29, SE= 0.09, p<0.001), and cues to action (b= 0.34, SE= 0.13, p= 0.008) showed indirect positive effects on hypertension preventive behavior. Conclusion: Hypertension preventive behavior is positively and directly affected by perceived threat, perceived benefit, self, and cues to action. The preventive behavior is negatively and directly affected by perceived barrier. Perceived susceptibility, perceived seriousness, and cues to action indirectly and positively affect on hypertension preventive behavior.

Pemeriksaan laboratorium sebelum pemberian transfusi darah (pretransfusion testing) merupakan bagian yang sangat vital dalam kegiatan transfusi. Pada beberapa jenis uji pra transfusi membutuhkan suspensi sel darah merah. Pembuatan suspensi sel bertujuan untuk mengoptimalkan reaksi antigen-antibodi sehingga reaksi yang muncul dapat diamati dengan jelas. Praktek kerja penentuan golongan darah menggunakan suspensi sel darah merah, di mana dalam penyediaannya menggunakan fase pencucian yang memakan waktu. Jenis sampel yang digunakan dapat berupa whole blood dengan antikoagulan ataupun sampel darah beku. Karena keterbatasan waktu, maka modifikasi dilakukan pada prosedur baku. Penelitian ini bertujuan untuk mengetahui perbedaan derajat aglutinasi uji golongan darah berdasarkan teknik penanganan sampel dalam pembuatan suspensi sel darah merah. Uji golongan darah Cell Grouping metode tabung dilakukan pada sampel darah vena dengan teknik penanganan sampel yang berbeda yaitu, sampel darah beku tanpa antikoagulan dengan penyimpanan, sampel darah segar dengan langsung penambahan NaCl 0,9%, dan sampel darah  antikoagulan EDTA dengan penyimpanan dalam refrigerator 8 – 24 jam dengan suhu 40C. Jumlah sampel untuk tiap jenis penanganan ada 30 sampel, dan dilakukan penilaian derajat aglutinasi pada uji golongan darah, dilakukan oleh 2 analis. Hasil mean derajat agutinasi dibedakan antara 3 teknik pembuatan suspensi dengan sampel NaCl (Mean= 3.88; SD= 0.32), sampel beku (Mean= 3.93; SD= 0.25), dan sampel EDTA (Mean= 3.98; SD= 0.13), namun perbedaan mean tersebut secara statistik tidak signifikan (p>0.05), sehingga dapat disimpulkan ketiga jenis sampel bisa digunakan untuk menggantikan satu dengan lainnya sebagai alternatif sampel yang sebanding dalam pembuatan suspensi sel darah merah untuk menentukan  golongan darah ABO metode cell grouping.