fied with inactive inosite, has never been spoken of as "a bios." It is one of the constituents into which Lucas separated Wildiers' bios; by itself it has little if any effect on the yeast crop; but if the other constituent, namely, "Bios II," be present in the culture medium, addition of inosite much increases the crop. As to the inosite from tea, Miss Eastcott's paper, J. Phys. Chem., 32, 1094 (1928, states that it was recrystallized from methyl alcohol and water and obtained in two "clear white" crystalline forms, anhydrous and the dihydrate, both of which were analyzed; also that it made no difference in the yeast crops obtained with various preparations of Bios II whether Kahlbaum's inosite was employed or that from tea. Her laboratory note-books show that the last six recrystallizations had no effect on the "activity" of the latter.
Variable-speed motor J. Trap (100-cc. Erlenmeyer) K. Three-way stopcock L. Gas-washing bottle for absorbing hydrogen sulfide filled with ammoniacal cadmium chloride solution M. Gas inlet tube
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