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Experiment was conducted on fish, Oreochromis mossambicus to study the effect of acetamiprid on tissue biomarker lactate dehydrogenase. Acetamiprid is a neonicotinoid pesticide and it is used by the farmers to protect their crops. This pesticide residue reaches to the aquatic ecosystem by many ways and affects the aquatic fauna. Lethal Concentration (LC 50) of acetamiprid for O. mossambicus has been calculated by probit analysis and recorded as 5.99 ppm at 96 h. Chronic exposure shows increased activity of LDH in liver, brain and gill tissues during all the exposure periods when compared with the control. This significant increase in enzyme activity was observed due to toxic effect of acetamiprid. Long term exposure of organisms to pesticides means a continuous health hazard for the fish population and it is on high risk by consuming these toxicated fishes.

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