We report on a simple experimental scheme to generate and control the orbital angular momentum (OAM) spectrum of the asymmetric vortex beams in a nonlinear frequency conversion process. Using a spiral phase plate (SPP) and adjusting the transverse shift of the SPP with respect to the incident Gaussian beam axis, we have transformed the symmetric (intensity distribution) optical vortex of order l into an asymmetric vortex beam of measured broad spectrum of OAM modes of orders l, l − 1, l − 2, …, 0 (Gaussian mode). While the position of the SPP determines the distribution of the OAM modes, we have also observed that the modal distribution of the vortex beam changes with the shift of the SPP of all orders and finally results in a Gaussian beam (l = 0). Using single-pass frequency doubling of the asymmetric vortices, we have transferred the pump OAM spectra, l, l − 1, l − 2, …, 0, into the broad spectra of higher order OAM modes, 2l, 2l − 1, 2l − 2, …, 0 at green wavelength, owing to OAM conservation in nonlinear processes. We also observed an increase in single-pass conversion efficiency with the increase in asymmetry of the pump vortices producing a higher power vortex beam of mixed OAM modes at a new wavelength than that of the pure OAM mode.

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Two-photon microscopy (TPM) offers deeper imaging depth inside the scattering medium, however, it suffers from limited resolution owing to the longer excitation wavelength. We demonstrate the use of a hollow Gaussian beam (HGB) at the therapeutic window to improve the resolution and signal-to-background ratio (SBR). The HGB was produced by omitting the azimuthal phase term from the vortex mode, and the excitation point spread function (PSF) can be readily tuned by the mode order. The performance of the TPM with HGB was evaluated by experimentally imaging 100 nm fluorescent beads to estimate the PSF. The HGB improved the lateral resolution of the TPM by 36% in contrast to the conventional TPM. The HGB also furnishes an improvement of SBR by eliminating the out-of-focus light owing to its ring shape. Furthermore, we have used a translating lens-based module for additional lateral resolution tuning and reduced the resolution further down to 44% with respect to conventional TPM. Finally, we have performed imaging with merely two-dimensional scanning of a 50 µm thick mouse brain slice (Thy-YFP H-line) using the developed TPM with HGB. Our compact, robust, and low-cost design of the HGB generation scheme can easily be integrated into the commercial TPM to accommodate the improvements.

Non-interferometric quantitative phase imaging at 1 μm wavelength regime is demonstrated using transport-of-intensity equation-based phase retrieval algorithm and fiber laser. Amplitude and phase information of the complex field of the sample has been retrieved and presented.

Phase sensitive two-photon microscopy is demonstrated using transport-of-intensity equation-based phase retrieval algorithm. Amplitude and phase information of the complex field of two-photon microscopy signal has been retrieved for fluorescence bead and presented.