Uji fitokimia kualitatif menunjukkan biji kelengkeng mengandung senyawa bioaktif flavonoid, glikosida, saponin dan tanin, sehingga berpotensi sebagai antibakteri. Sedangkan nanokitosan, selain memiliki aktivitas antibakteri, dapat juga dijadikan penghantar obat-obatan dan ekstrak tanaman. Penelitian ini bertujuan membandingkan perbedaan efektivitas campuran nanokitosan 1% dengan ekstrak biji kelengkeng konsentrasi 80%, 60%, 40% dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Pembuatan ekstrak biji kelengkeng dilakukan dengan metode maserasi dan dibuat dalam konsentrasi 80%, 60%, 40%. Adapun nanokitosan 1% dibuat dengan metode gelasi ionik. Kedua bahan tersebut dicampurkan dengan perbandingan 1:1. Uji antibakteri terhadap campuran ekstrak dan nanokitosan tersebut menggunakan metode difusi cakram. Hasil penelitian menunjukkan mean daya hambat dari campuran konsentrasi 80% adalah 9.38 mm; konsentrasi 60% (8,47 mm) dan konsentrasi 40% (7,10 mm). Uji statistik one way ANOVA menunjukkan bahwa campuran yang paling efektif dalam menghambat Staphylococcus aureus terlihat pada konsentrasi 80% dibandingkan konsentrasi 60% maupun 40%, dimana p=0.000 (p<0.05). Hasil penelitian ini menunjukkan pertambahan konsentrasi ekstrak biji kelengkeng meningkatkan daya hambat terhadap Staphylococcus aureus. Hal ini disebabkan oleh adanya peningkatan jumlah kandungan senyawa aktif dari ekstrak biji kelengkeng seiring dengan pertambahan konsentrasi sehingga meningkatkan efektivitasnya dalam merusak sel Staphylococcus aureus.

Nowadays, many Indonesian people return to various traditional medicinal plants to cure various diseases. This is due to the development of various sciences and technology, one of which is in the Atsiri field. Essential oils are often used as aromatherapy because they can foster a feeling of calm and can keep away from feelings of anxiety and restlessness. The use of essential oils from spices is a suitable choice of innovation in today's modern world of health. The purpose of this research is to make aromatherapy products using high-grade patchouli oil and essential oils of Acehnese spices that are efficacious for treating various diseases. The ingredients used in the manufacture of this product are high-grade patchouli oil, cajuput oil, nutmeg oil, cinnamon oil, clove bud oil, menthol, champers, and carrier oil. The research stage was started by developing the aromatherapy formulas, establishing the working procedures, and conducting quality testing. This spice aromatherapy product was tested on 25 respondents to determine its effectiveness on health. The results show that 68% respondents agree that spice aromatherapy products can affect body relaxation and 55% agree that they can improve sleep quality. As many as 88% of respondents agree that spice aromatherapy is effective for reducing headaches and catching colds and 84% of respondents agree that this product has an effect on relieving breathing. The results also show that all respondents agree that this product has a unique and classic aroma. However, there are some respondents (28%) who do not like this scent, while 72% really like it. These results show that spice aromatherapy products still need to be formulated to get other aroma variants to provide an alternative for consumers who do not like the strong aroma of spices.

Relaxation is considered as a process that relieves stress on your mind and body. At the present,  there are many different forms of relaxation and aromatherapy. One of the important products to  be developed further is aromatherapy candles. However, candles on the market generally still use  materials that are not environmentally friendly such as paraffin. So this study aims to making  aromatherapy candle using natural ingredients from essential oils and soybean oil. Soybean oil (Soy wax) is a potential substitute for paraffin which is biodegradable, renewable and friendly  environment. This study also uses patchouli oil, peppermint oil and lemon oil which have benefits  for anti-depressant, relieve pain and for immune system. The aromatherapy candle materials consist  of : soy wax, essential oils, dyes, candle wick and candle glass. The research stage was started by  developing the aromatherapy candle formulas, establishing the working procedures, and  conducting quality testing, involve test the preference for the smell of candles before and after  burning, burn time test, test for the overall appearance of the candle, visual test colour preferences  and therapeutic effect. The research revealed that the aromatherapy candle 1) 73,3% respondent agree the aroma of the candle more preferable when the candle was burned compared to unburned  candle; 2) have burn time ranged from 8 hours 14 minutes to 10 hours 20 minutes; 3) 93.3% of  respondents like and really like the texture and visual of the candle, based on SNI 0386-1989-A/II  0348-1980, the physical condition of a good candle is to have the same and even colour, not cracked,  not deformed and not broken; 4) 80% respondent like and really like the colour of the candle; 5)  93.3% respondent agree and strongly agree that the aroma of candles has a good therapeutic effect for provide a calm, comfortable effect and can improve mood.