The use of herbal medicine is increasingly becoming more accepted in treatment of many inflammatory conditions. Omega-3 is recently introduced as an effective therapeutic agent and a powerful anti-inflammatory agent, which promotes whole-body wound healing including ulcers as it has an important role in modulation of proinflammatory cytokine production. This study was conductedto evaluate and compare the effect of topicalapplication and systemic administration of omega-3 on the healing of oral mucosal wounds which wereexperimentally induced in albino rats. The experiment comprised 45 albino rats which were divided into 3 groups (n= 15 per group); control group not receiving any treatment(C) group (L) receiving topical application of omega3, and group(S) receiving systemic omega3; 5 rats from each group were sacrificed at day 2, 4 and 8 respectively. Tissue samples were obtained and prepared for histological and biochemical evaluations. The data obtained were analyzed using one way ANOVA and Post Hoc Tukey statistical analysis with the previously performed tests of normality and homogeneity. The biochemical results showed significant improvement regarding TNF-α and IL8-levels after 4 and 8 days in both groups compared to the control. The topical omega3 group showed better results than the systemic with significant differences at 4& 8 days inTNF-α while for IL8 the difference was only significantafter 8 days. The histological results revealed severe epithelial degeneration and inflammation in the lamina propria after 2 days whether using local or systemic treatment however increase reepithelialization gradually increased at 4 days and an even thickness of the epitheliumwas noted by the end of day 8. Conclusion:Omega 3 greatly enhanced ulcer healing and showed superior effects using topical application.

Oral proliferative verrucous leukoplakia (OPVL) is a rare form of oral leukoplakia, which was first described in 1985 by Hansen et al, it is characterized by a distinct clinical form and defined by its progressive clinical course, changing clinical and histopathological features, and potential to develop into cancer usually higher than 70%. OPVL is observed more frequently in elderly women, over 60 years at the time of diagnosis. The buccal mucosa and tongue are the most frequently involved sites. It develops initially as a white plaque of hyperkeratosis that eventually becomes a multifocal disease with confluent, exophytic and proliferative features with a progressive deterioration of the innocuous white oral lesions that it can resemble clinically. Characteristic histological features are lacking, and the diagnosis is based on the combination of clinical and histological findings showing progression of the lesion. Case ReportWe hereby report a case of 55 years old female patient having oral proliferative verrucous leukoplakia (OPVL), affecting different oral sites: lateral border of the tongue, gingival and buccal mucosae. Clinical intraoral examination revealed multifocal thick stiff white patches that could not be wiped off, differential diagnosis was made. Histological examination showed hyperkeratosis, acanthosis and dysplastic changes but there was not any frank invasion, a diagnosis of OPVL was made.

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Omega-3 was recently introduced as an effective therapeutic agent and a powerful antiinflammatory agent, which promotes whole-body wound healing including ulcers as it has an important role in modulation of proinflammatory cytokine production. Aim of study: This study was conducted to evaluate and compare the effect of topical application and systemic administration of omega-3 on the healing of oral mucosal wounds which were experimentally induced in albino rats. Materials & Methods: The experiment comprised 45 albino rats which were divided into 3 groups (n= 15 per group); control group (C) not receiving any treatment, group (L) receiving topical application of omega-3, and group (S) receiving systemic omega-3, 5 rats from each group were sacrificed at day 2, 4 and 8 respectively. Tissue samples were obtained and prepared for histological and biochemical evaluations.The data obtained were statistical analyzed and compared. Results: Biochemical results showed significant improvement regarding TNF-α and IL-8 level after 4 and 8 days in local and systemic groups compared to the control. The topical omega-3 group showed better results than the systemic with significant differences in TNF-α after 2 and 4 days, while for IL-8 the difference was only significant after 8 days. The histological results in both local and systemic treated groups after 2 days revealed inflammation and epithelial degeneration which gradually reepithelized by 4 to 8 days. Conclusion: Omega-3 greatly enhanced oral ulcer healing and showed superior effects using topical application.

Background: Over the years, numerous studies proposed a crucial role of mutations of nuclear DNA in the carcinogenesis process. Of late, many researchers suppose that alterations of mitochondrial DNA should not be excepted from this analysis. Mutational analysis of mitochondrial DNA displayed that mitochondrial D-loop is assessed as a hotspot for molecular alterations in various types of malignant tumors encompassing oral squamous cell carcinoma. Squamous cell carcinoma is believed to emerge through precancerous stages, which might be merely morphologic aspects of cumulative genetic variations. Methods:In keeping with this model of molecular tumor progression, this study aimed to investigate the qualitative and quantitative alterations that might occur in mitochondrial D-loop in oral leukoplakia whether dysplastic or not by semiquantitation of a product of the polymerase chain reaction and sequence analyses of mitochondrial D-loop gene.Results: Statistically significant increases in the mean values of D-loop concentrations were observed across the dysplasia gradient of oral leukoplakia.Sequence analyses revealed the presence of point mutations in both dysplastic and nondysplastic oral leukoplakia but not in normal mucosa. Conclusion:The results of this study suggested that quantitative and qualitative alterations in mitochondrial D-loop could be a promising molecular marker for early detection and progression of the malignancy. K E Y W O R D S mitochondrial D-loop, mutations, oral leukoplakia, polymerase chain reaction J Cell Biochem. 2019;120:13944-13951. wileyonlinelibrary.com/journal/jcb 13944 |