Background The present study aimed to explore the effect of 8 weeks of concurrent aerobic-resistance training on thyroid stimulating hormone (TSH), thyroxine (T4), blood lipid profile, and blood glucose hemostasis in adolescent girls with hypothyroidism. Materials and methods In this clinical trial, 20 adolescent girls with hypothyroidism [age: 12.10 ± 1.94 years; body mass index (BMI): 25.25 ± 2.91 kg/m2] were selected and randomly assigned to experimental and control groups. The experimental group was treated with 8 weeks of concurrent aerobic [60–80% heart rate reserve (HHR)] and resistance (40–65% of 1RM) training. Blood was sampled before and after training. Data were analyzed using a pairwise t-test and an independent t-test (α < 0.05). Results Within-group comparison revealed significant decline of body index, BMI, fat percentage, fasting blood sugar, and homeostatic model assessment (HOMA) in the experimental group (p = 0.002, p = 0.001, p = 0.001, p = 0.03, and p = 0.02, respectively). Also, inter-group comparisons indicated significant differences in weight, BMI, fat percentage and HOMA (p = 0.001). But, no significant difference was observed in the blood sugar index (p > 0.05). Conclusion A short-term period of concurrent aerobic-resistance training may improve blood glucose hemostasis and body composition of adolescent girls with hypothyroidism. However, further research is necessary.