A new spectrophotometric method has been developed for the assay of olanzapine (OLN.) in pure and dosage forms. The method is based on the diazocoupling of (OLN.) with diazotized p-nitroaniline in alkaline medium to form a stable brown colored water-soluble azo dye with a maximum absorption at 405 nm.  The variables that affect the completion of reaction have been carefully optimized. Beer’s law is obeyed over the concentration range of (0.5-45.0 μg.mL-1) with a molar absorptivity of 1.5777×104 L.mol-1.cm-1.  The limit of detection was  0.3148  Î¼g.mL-1 and  Sandell’s  sensitivity value was 0.0198 μg.cm-2. The proposed method has been applied successfully to the determination of olanzapine in tablet pharmaceutical preparations. Keywords: Spectrophotometry, Olanzapine, Diazotization, p-nitroaniline, tablet dosage form.

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

A recommended, simple and sensitive method for the spectrophotometric determination of diclofenac sodium drug (DLC) in pharmaceutical formulations was represented in this work. The method is based on the coupling of DLC with diazotized 4-Aminoacetophenone in basic medium to form an intense yellowish-brown colored diazocoupling product that showed maximum absorbance at 362 nm. The effect of various factors such as amount of the reagent, time of the reaction and order of addition were investigated. The experimental results indicated a good linearity (r 2 = 0.9954) over a concentration range of (2-40) µg.mL -1 with a detection limit of 0.447 µg.mL -1 diclofenac sodium under the optimized conditions. The pharmaceutical excipients did not exhibit any interferences. The low value of the standard deviation alongside good correlation coefficient confirms the applicability of the method. Accordingly, the proposed method has been successfully applied to the determination of diclofenac sodium in pharmaceutical formulations. The results were statistically compared with standard methods by means of t-test and f-test at 95% confidence level with no significant differences observed.

The reaction of 2- (1, 1-dimethyl-1, 3-dihydro-2H-benzo[e]indol-2-ylidene) malonaldehyde with various substituted o-phenylinediamine resulted in a number of new indole Schiff base derivatives. Total leukocyte count (TLC), Fourier-transform spectroscopy (FTIR), Proton nuclear magnetic resonance (1HNMR), characterized the chemical structures of the synthesized compounds and quantitatively estimated using FT-IR spectroscopy, the results showed linear in concentration ranges 10.0 to 50.0 ppm. The biological activity of some novel synthesized compounds was tested against two types of bacteria. Compounds exhibit antibacterial action, according to the provided data.

A study aimed to recommend spectrophotometric method for the determination of clonazepam (CLZ.) in pure form and their pharmaceutical tablets by the reaction of sited drug with ferric chloride in presence potassium ferric cyanide in acid medium to form prussian blue complex and determine it by UV-Vis spectrophotometric at 526 nm. The variables that affect the completion of reaction have been carefully optimized. The linearity range was (2-35μg. mL -1 ) with a molar absorptivity of 2.8035×10 4 L.mol -1 . cm -1 . The limit of detection was 0.5703μg. mL -1 and Sandell's sensitivity value was 0.0103μg. cm -2 . The suggest method was successfully used to estimate clonazepam in commercial formulations.