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ObjectivesIn order to explore Coffea canephora green seeds as natural extract for application in the functional-food industry, we focused this study to the evaluation of the antioxidant and the antiadhesion effect of C. canephora green seeds extracts.MethodsThe analysis of C. canephora green seeds extracts was carried out by RP-HPLC-PDA. These extracts were screened for antioxidant activities by ABTS and phenanthroline assays. The antibacterial activity was determined by microdilution method against three reference bacteria. The inhibition of bacterial adhesion at 1/8 MIC was carried out against three reference bacteria.ResultsThe RP-HPLC-PDA revealed the presence of gallic acid, vanillin, quercetin, chlorogenic acid, and P-coumaric acid. The n-buatnol extract have the highest activity ABTS assays (3.96 ± 0.08 μg/mL). For this extract, the A0.5 was 1.90 ± 0.05 μg/mL for phenanthroline assay. The n-butanol extract and the methanolic extract have the higher antibacterial activity against Staphylococcus aureus ATCC 25923 (40 µg/mL).At MIC/8, the extracts of C. canephora showed 70% higher antidhesive activity against S. aureus ATCC 25923.ConclusionsOur finding provides an effective and specific new approach to the search of antioxidant and antiadhesive compounds for different uses.

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Background: Glycyrrhiza glabra L. is an important medicinal plant endowed with various pharmacological virtues. This study aimed to investigate the antioxidant, antibacterial, and anticholinesterase activities of the Algerian Glycyrrhiza glabra L. roots extracts. Methods: The chemical composition of both chloroformic (LCh) and ethyl acetate (LAE) extracts were analyzed by RP-HPLC-PDA and 1H NMR spectroscopy. The antioxidant activity was evaluated using hydrogen atoms transfer methods (DPPH) and single electron transfer (ABTS and CUPRAC assays). The antibacterial activity was realized against different strains via the Minimal Inhibitory Concentration (MIC), when the anticholinesterase activity was performed through the acetylcholinesterase and butyrylcholinestrase enzymes inhibition. Results: The chemical analysis revealed the presence of phenolic acids (gallic acid, p-coumaric acid) and a hydroxycinnamic compound (ferulic acid). However, flavonoids were represented by quercitin, rutin (flavonol), and glabridin (isoflavane). The 1H NMR of the L4 fraction from LCh extract allowed to characterize the structure of glabridin. The antioxidant assays revealed that LCh extract is the best among the others extracts with IC50 DPPH of 33.94 µg/mL, IC50 ABTS of 3.45 µg/mL and CUPRAC A0.5 of 21.78 µg/mL. The LCh extract displayed an effective antibacterial activity with MIC’s of 19.5 µg/mL against seven gram positive and negative bacteria strains. The same extract showed a potent butyrylcholinestrase inhibitory activity with IC50= 4.72 ± 0.72 µg/mL, which is too strong then the standard drug. Conclusion: The study demonstrated that G. glabra root extracts had a high antibacterial, and free radical scavenging. It was also able to inhibit cholinesterase enzymes, which confirm the effectiveness of phytoconstituents present in the plant especially flavonoids.