Corona viruses are a family of viruses that can cause diseases such as the common cold and acute respiratory infection and in 2019, a new type of corona virus was discovered that caused an outbreak of a disease that originated in China. The virus is known as severe acute respiratory syndrome corona virus (SARS-CoV-2). The resulting disease is called emerging corona virus disease 2019 (COVID-19). In March 2020, the World Health Organization declared the corona virus (COVID-19) to be a global pandemic. In this research, a diagnostic kit was prepared that is used in the laboratory to detect infection with Acquired Corona Virus (COVID19) by the method of the enzyme immunoassay (ELISA). Conjugated secondary antibodies tagged with HRP which gives a color signal with the substrate added to it, its intensity depends on the amount of antibodies present in the pathological sample. We used a microtiter plate coated with the virus core and ns antigen and a conjugate product from Imbian Company, while the other of the kit components (reagents and buffers) were prepared in Al-Razi laboratories to be suitable for use. Tests were conducted on the prepared kit for 96 samples, including 55 samples for positive cases and 41 samples for negative cases, which were obtained from the specialized laboratories and patients. The tests showed conformity in the results compared to foreign kit used for this purpose and using the ELISA washer and reader devices available in Al-Razi center's laboratories. And by installing the method of preparation by fixing the method of preparation and obtaining identical results, Al-Razi center can produce pioneering batches and provide the laboratories of Ministry of Health of this type of diagnostic kits.