Probiotics are beneficial microorganisms, and the evaluation of their safety for human use in the food industry has become critical. This study examines the safety of Bacillus coagulans IDCC 1201 isolated from green malt by analyzing its genomic and phenotypic characteristics and determining its toxicity. The presence of antibiotic resistance and toxigenic genes and gene transferability were investigated using whole-genome analysis. The strain's hemolytic and enzyme activities, minimum inhibitory concentrations of antibiotics, and biogenic amine and D-lactate production were also examined. Furthermore, the principal properties of B. coagulans IDCC 1201 as probiotics, such as resistance to abiotic stress and intestinal adhesion, were studied. The whole-genome analysis demonstrated that B. coagulans IDCC 1201 had no antibiotic resistance or toxigenic genes; the strain was susceptible to the nine antibiotics proposed by the European Food Safety Authority. Moreover, this strain lacked hemolytic and β-glucuronidase activities. Additionally, it was confirmed that B. coagulans IDCC 1201 produced undesirable metabolites, including biogenic amines or D-lactate, at a safe level. Finally, the strain exhibited functional potential as a probiotic in terms of abiotic tolerance, such as bile tolerance and intestinal adhesion in in vitro experiments. In conclusion, B. coagulans IDCC 1201 can be considered as a safe probiotic with regard to human health.

Lactic acid bacteria have been considered safe and commonly used in foods and fermentation processes (Bernardeau et al., 2006; Zielinska & Kolonzyn-Krajewska, 2018). Typically, they are Grampositive, mostly nonmotile, nonspore-forming, facultative anaerobic (or microaerophilic), and rod-(or cocci-) shaped bacteria that are utilized in fermented dairy and nondairy products such as fermented vegetables, meats, and beverages (Nuraida, 2015). Although the majority of probiotics contain Lactobacillus and Bifidobacterium, Streptococcus genus is one of the most widely used probiotic strain. Streptococcus thermophilus has been generally isolated from traditional fermented milk and yogurt products (Vendramin et al., 2017; Zhang et al., 2019). In particular, S. thermophilus has been used as a starter for dairy products because of rapid acidifying capacity during fermentation process (Naumenko et al., 2019; Santos et al., 2019). It has been also reported to exhibit several health-beneficial effects such as growth inhibition of many pathogenic bacteria and strong adherence to the gastrointestinal tract (Braun et al., 2020; Iyer et al., 2010; Wu et al., 2014). Although probiotics have been regarded as GRAS (generally recognized as safe) strains, recent significant developments in the field

Purpose Lactobacillus reuteri is one of the most largely studied human-commensal bacteria and widely used as a form of probiotics. Safety of probiotics has become increasingly important for human consumption due to increasing health-concerns in food industry. In this study, the safety of L. reuteri IDCC 3701 isolated from human breast milk was thoroughly investigated. Methods Whole-genome sequence analysis was performed to identify antibiotic resistance and toxigenicity of L. reuteri IDCC 3701. Phenotypic analysis such as minimal inhibitory concentration, β-hemolysis, extracellular enzyme activity, and the production of biological amines and L/D-lactate, was investigated. Finally, acute oral toxicity test was performed to access L. reuteri IDCC 3701 safety. Results Although multiple resistances to gentamicin and kanamycin were observed in L. reuteri IDCC 3701, it was revealed that these resistances are intrinsic and not transferable through whole-genome analysis. In addition, various phenotypic analysis concerning hemolysis, enzyme activity, and D-lactate production did not show any negative results. Although L. reuteri IDCC 3701 harbors a histidine decarboxylase gene, no biogenic amines were detected. Finally, L. reuteri IDCC 3701 exhibited no evidence of acute toxicity according to an in vivo study. Conclusion Our findings demonstrate that L. reuteri IDCC 3701 is considered to be safe for human consumption as probiotics based on the in silico, in vitro and in vivo studies.

A battery-charged electric-vehicle (EV) technology has recently been developing fast due to energy efficiency and environmental problems. To commercialize these EVs, the EV charging infrastructure should be completely installed. Power line communication (PLC) is a technology designed to use the existing power lines and support communications. As such, its application is under study in diverse fields such as in the outdoor, in-home, in-plane, in-ship, and in-vehicle scenarios. An ISO/IEC 15118 standard is pushing to standardize PLC as a technology that supports EV charging systems. To apply PLC to the EV charging system, this paper investigates the EV and EV charging structures and analyzes requirements for applying PLC. The PLC modem performance was also tested with the charged EV.

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