No abstract

Penyakit degeneratif seperti kanker, penuaian dini dan jantung dapat disebabkan oleh adanya radikal bebas. Radikal bebas memiliki satu rantai yang tidak memiliki pasangan electron sehingga sangat reaktif untuk mencari pasangan electron pada sel yang normal. Ikatan ini dapat menganggu sinyal sel sehingga terjadilah kerusakan sel pada sel normal. Radikal bebas dapat ditangkal dengan adanya bahan antioksidan. Melihat kandungan polifenol dan flavonoid pada propolis pada penelitian sebelumnya, maka ditelitilah aktivitas antioksidan dari propolis ini. Penelitian ini bertujuan untuk menentukan besarnya aktivitas ekstrak etanol propolis berdasarkan dari nilai IC50 (Inhibition Consentrasi) menggunakan metode DPPH. Ekstrak etanol propolis dibuat dalam konsentrasi 400 µg/ml, 800 µg/ml, 1200 µg/ml, 1400 µg/ml, 1600 µg/ml, 1800 µg/ml dan 2000 µg/ml sedangkan vitamin C dibuat dalam konsentrasi 10 µg/ml, 20 µg/ml, 30 µg/ml, 40 µg/ml, dan 50 µg/ml. Masing-masing seri ditambahkan DPPH 40 µg/ml dan diinkubasi selama 30 menit. Sampel diukur pada spektrofotometer UV-Vis pada panjang gelombang 516 nm. Nilai IC50 yang diperoleh adalah 1216.66 µg/ml dan nilai IC50 Vitamin C adalah 13.7658 µg/ml. Dari hasil ini menunjukkan bahwa ekstrak etanol propolis termasuk dalam kategori tidak aktif dibandingkan dengan vitamin C. Kata Kunci : Antioksidan, IC50 , Ekstrak Etanol Propolis, DPPH, Vitamin C

Data shows that the success of the Directly Observed Treatment Shortcourse program(DOTS) in South Sulawesi Province was 74.8%. Causes of failure include standard drug side effects, length of treatment period and Mycobacterium tuberculosis (M.tb) bacterial resistance. The development of new drugs today in addition to synthetic chemical drugs are also natural materials that have the potential as antituberculosis with various mechanisms. The aim of the research is to prove the potential of plants used by the community as tuberculosis drugs empirically based on phytochemical screening extracts, M.tb sensitivity to extracts and determine the MIC (Minimum Inhibitory Concentration) and MKC (Minimum Killing Concentration) values of M.tb. Selection of plant test materials based on survey results and Riskesdas data in 2015.Testing the potential of plant extracts based on the sensitivity of M.tb to the test material in vitro with an incubation period of 7 weeks. Showed 25 plants that have been used empirically as tuberculosis drugs proved to be potential antibacterial M.tb. Phytochemical screening produces flavonoids and tannins in all extracts. Some extracts contain alkaloids, saponins and steroids. Potential tests show that the extract concentration that is effectively used as a tuberculosis drug is: Meniran leaves and miana 100 ppm. Banana peel and basil leaves 125 ppm. 150 ppm bangle rhizome. Turmeric rhizome 175 ppm. Roselle flowers, soursop leaves and lime 200 ppm. Sandalwood, bitter melon leaves and binahong 225 ppm, Curcuma rhizome, kencur, turmeric and onion tubers, gotu kola leaves, sea lava and papaya 275 ppm. Ginger rhizome, brotowali stem, noni leaf, cloves and mangosteen peel > 275 ppm. 90% garlic bulb extract.

Dengen (Dillenia serrata) is an endemic plant found in Luwu Regency. Its primary utilization is still limited to the fruit as a food ingredient, such as dodol, though leaves and barks are often used as medicine. According to Santi , dengen leaves and bark contain large polyphenols. This study determines the sunscreen activity of the Dengen plant, specifically leaves and bark obtained from Malangke City in Luwu Regency. Also, the study aims to establish the part of the plant with great potential for sunscreen. The leaves and bark of the tree are extracted by maceration using 70% ethanol solvent. The determination of the SPF value was carried out in vitro based on the principle of measuring extraction absorption with a certain concentration at wavelengths of 290-320 nm. The ethanol extract of the bark is made with a concentration series of 100, 200, 300, 400, and 500 ppm, where 100 ppm produced an SPF value of 4,611. Furthermore, the ethanol extracts of the leaves were made in series 50, 100, 150, 200, 250 ppm, where 100 ppm produced an SPF value of 2. This study concluded that the ethanol extract of dengen bark has maximum sunscreen activity with a low concentration compared to ethanol leaves.

Degenerative diseases such as cancer, heart disease is one of the diseases caused by free radicals. Dengen (Dillenia serrata) is one of Indonesia's endemic local plants that has the potential to bind free radicals. So far, only limited to the use of fruit and consumed directly. This study aims to determine the ability of dengen stem bark extract to bind to free radicals expressed in IC50. In the initial research, the total content of polyphenols in dengen stem bark extract was 444.8 mg GAE / g or 44.48%. Dengen stem bark is extracted by maceration using 70% ethanol solvent. Antioxidant activity was calculated based on IC50 (Inhibition Concentration) values using the DPPH method, and measured at a wavelength of 516 nm. Ethanol extract of dengen bark is made in 5 concentration series, namely 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. Ethanol extract of dengen bark has a very active antioxidant activity with an IC50 value of 48.33 ppm. Whereas for comparison vitamin C has an IC50 value of 15.448 ppm. From the results, it can be concluded that the ethanol extract of dengen bark has the ability to bind free radicals with IC50 48.33 ppm