The study was conducted to evaluate the effects of different extenders, cryoprotectants and glycerol additions on the postthaw fertility and interactions between extenders and cryoprotectants during cryopreservation. Semen was collected by abdominal stripping from 30 adult male rainbow trout (Oncorhynchus mykiss Walbaum, 1792) and diluted with three different extenders (Erdahl-Graham, Lahnsteiner, glucosebased) containing 15% DMA, 15% DMSO, 15% DMA + 1% glycerol and 15% DMSO + 1% glycerol at a ratio of 1 : 2. Diluted samples were frozen as 0.1 ml pellets directly on dry ice (solid CO 2 , )79°C). Eggs were pooled from 10 females. Fertilization was applied in plastic dishes and 600 eggs were used in each fertilization trial. Pellets were thawed in their own extenders (30°C) at a ratio of 1 : 10. 0.3% NaCl was used for activating motility. Sperm-egg ratio was approximately 3 · 10 6 sp per egg. Experimental success was determined as the percentage of eyed-eggs 25 days after fertilization. The highest eyed-egg rate (49.3%) was obtained from semen frozen with glucose-based extender containing 15% DMSO + 1% glycerol. Our results indicate that the glucose-based extender containing DMSO is a useful combination, but that the addition of glycerol does not have a positive effect on postthaw fertility, and that interaction of the extender-cryoprotectant is also important in the cryopreservation of rainbow trout semen.

No abstract

No abstract

As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusively as an on-line Open Access (OA) quarterly accessible by all AquacultureHub (http://www.aquaculturehub.org) members and registered individuals and institutions. Please visit our website (http://siamb.org.il) for free registration form, further information and instructions. This transformation from a subscription printed version to an on-line OA journal, aims at supporting the concept that scientific peer-reviewed publications should be made available to all, including those with limited resources. The OA IJA does not enforce author or subscription fees and will endeavor to obtain alternative sources of income to support this policy for as long as possible.

As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusively as an on-line Open Access (OA) quarterly accessible by all AquacultureHub (http://www.aquaculturehub.org) members and registered individuals and institutions. Please visit our website (http://siamb.org.il) for free registration form, further information and instructions. This transformation from a subscription printed version to an on-line OA journal, aims at supporting the concept that scientific peer-reviewed publications should be made available to all, including those with limited resources. The OA IJA does not enforce author or subscription fees and will endeavor to obtain alternative sources of income to support this policy for as long as possible.