In this work, an S hybrid nanosheet with multiple functions is synthesized by in situ modification of gold nanoparticles (AuNPs) onto two-dimensional (2D) metalloporphyrinic metal–organic framework (MOF) (Cu-tetra­(4-carboxyphenyl)­porphyrin chloride­(Fe­(III)), designated as AuNPs/Cu-TCPP­(Fe). Cu-TCPP­(Fe) nanosheets contribute peroxidase-like activity, and AuNPs have glucose oxidase (GOx) mimicking performance, which induce the cascade catalysis reactions to convert glucose into hydrogen peroxide (H2O2), and then, by using AuNP catalysis, H2O2 oxidizes the no Raman-active leucomalachite green (LMG) into the Raman-active malachite green (MG). Simultaneously, in the presence of AuNPs, sensitive and selective surface-enhanced Raman scattering (SERS) determination of glucose can be achieved. The bioenzyme-free SERS assay based on such AuNPs/Cu-TCPP­(Fe) nanosheets is used for detection of glucose in saliva, showing good recovery from 96.9 to 100.8%. The work paves a new way to design a nanozyme-based SERS protocol for biomolecule analysis.

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Creatinine level in urine is an important biomarker for renal function diseases, such as renal failure, glomerulonephritis, and chronic nephritis. The Au@MIL-101(Fe) was prepared by in situ growth of Au nanoparticles in MIL-101(Fe) as a selective SERS substrate. The Au@MIL-101(Fe) offers the great local surface plasmon resonance (SPR) effect due to gold nanoparticles aggregation inside metal-organic frameworks. The framework structure could enrich trace target samples and drag them into SPR hot spots. The optimal Au@MIL-101(Fe) composite substrate is used for analyzing creatinine in urine and the limit of detection is down to 0.1[Formula: see text][Formula: see text]mol/L and a linear relationship is ranging from 1[Formula: see text][Formula: see text]mol/L to 100[Formula: see text][Formula: see text]mol/L.

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